| 黄 慧1, 张晓勇1, 郑 欢1, 蔡青苡1, 罗佳佳1, 罗 凯2, 李树江1, 杨友联1*.羊肚菌菌盖干腐病病原菌鉴定及培养特性研究[J].植物保护,2022,48(1):66-72. |
| 羊肚菌菌盖干腐病病原菌鉴定及培养特性研究 |
| Identification and cultural characterization of Diplo spora longispora associated with pileus rot disease on cultivated morel |
| 投稿时间：2020-10-13 修订日期：2020-11-27 |
| DOI：10.16688/j.zwbh.2020537 |
| 中文关键词: 羊肚菌 菌盖干腐病 长孢卵单隔孢霉 系统鉴定 培养特性 |
| 英文关键词:morel pileus rot disease Diplo spora longispora phylogenetic analysis cultural characteristics |
| 基金项目:贵州省特色重点实验室建设项目(黔教合KY 字［2017］012);2019年贵州省大学生创新创业训练计划(20195201506);六盘水市科技计划(520202016HK07，52020-2018-03-07);2018年度六盘水师范学院项目“微生物学教学团队”(LPSSYjxtd201802) |
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| 中文摘要: |
| 菌盖干腐病是羊肚菌 Morchella spp.出菇后发生最严重的病害之一, 常给羊肚菌生产造成较大损失。为明确引起贵州六盘水地区羊肚菌菌盖干腐病致病菌种类, 采用单孢分离法从六盘水地区3个羊肚菌栽培点的菌盖干腐病样品上分离到9个菌株。基于形态学及ITS和LSU基因序列分析将9个菌株鉴定为长孢卵单隔孢霉 Diplo spora longispora。培养特性研究表明, 该菌最适的培养基为PCA, 最适氮源为蛋白胨与硝酸铵, 最适碳源为葡萄糖与麦芽糖, 菌丝生长及孢子萌发最适温度为15~25℃, 最适pH为6~10;当温度超过30℃菌丝即停止生长, 孢子无法萌发。 |
| 英文摘要: |
| Pileus rot disease widely occurs and often causes serious yield losses of cultivated morel. In order to identify the pathogen of pileus rot disease, nine strains were obtained by single spore isolation in three cultivation sites of Liupanshui city. Based on morphological characteristics and ITS and LSU sequence analysis, the nine strains were identified as the same species, Diplo spora longispora . Cultural characterization showed that PCA was the optimal medium for colony growth, the optimal nitrogen sources were peptone, ammonium nitrate, and the optimal carbon sources were glucose and maltose. The best temperature and initial pH ranges for spore germination and mycelial growth were 15-25℃ and 6-10, respectively. When the temperature exceeded 30℃, the mycelium stopped growing and the spores failed to germinate. |
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