| 张乃元 1, 2, 张 蕾 2, 程云霞 2, 张宝琴 1*, 江幸福 2*.蜕皮激素对黑化黏虫体液免疫的影响[J].植物保护,2021,47(4):102-106. |
| 蜕皮激素对黑化黏虫体液免疫的影响 |
| Effects of molting hormone on humoral immunity of the black Mythimna separata (Walker) larvae infected by Beauveria bassiana |
| 投稿时间：2020-04-30 修订日期：2020-05-19 |
| DOI：10.16688/j.zwbh.2020225 |
| 中文关键词: 黏虫 黑化幼虫 蜕皮激素 20-羟基蜕皮酮 酚氧化酶活性 溶菌酶活性 |
| 英文关键词:Mythimna separata black larvae molting hormone 20E PO activity lysozyme activity |
| 基金项目:国家公益性行业(农业)科研专项(201403031); 国家绿肥产业技术体系(CARS-22); 国家自然科学基金(31672019, 31871951); 国家重点研发计划(2017YFD0201701, 2017YFD0201802); 北京市自然科学基金((6172030) |
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| 中文摘要: |
| 为揭示蜕皮激素(MH)对黑化黏虫免疫能力的影响, 本试验对被球孢白僵菌侵染后的黏虫体内MH含量的动态变化进行了分析比较, 并对被侵染的黑化黏虫注射20-羟基蜕皮酮(20E), 分析比较其注射20E后的死亡率以及酚氧化酶(PO)和溶菌酶活性的变化?结果表明:被白僵菌侵染后, 黑化黏虫6龄幼虫的MH含量在侵染后8 h( P <0.05)?72 h( P <0.05)和96 h( P <0.05)均显著高于对照(注射吐温-80)?注射球孢白僵菌后立即注射5 μg/μL的20E稀释液, 黑化黏虫的死亡率显著低于对照组(注射球孢白僵菌后注射无水乙醇)( P <0.05), 其原因是注射5 μg/μL 20E稀释液后, 被球孢白僵菌侵染后8 h和24 h的黑化黏虫体内PO和溶菌酶活性均显著高于对照组(注射球孢白僵菌后注射无水乙醇)( P <0.05)?试验结果表明MH在黏虫体液免疫反应中起到重要的调节作用? |
| 英文摘要: |
| In order to investigate the effect of molting hormone (MH) on the immune ability of the black Mythimna separata (Walker) larvae, the dynamic changes of MH contents in M. separata larvae infected by Beauveria bassiana were analyzed and compared. 20-hydroxyecdysone (20E) was injected into the infected black larvae of M. separata, and the dynamic changes of phenol oxidase (PO) and lysozyme activities after 20E injection were analyzed and compared. The results showed that, after being infected with B. bassiana, the MH content of the 6th instar black larvae was significantly higher than in the control at 8 h ( P <0.05), 72 h ( P<0.05) and 96 h ( P <0.05) after infection. M. separata infected with B. bassiana was injected with 5 μg/μL of 20E dilution, and the mortality rate was significantly lower than in the control (injecting anhydrous ethanol after being infected by B. bassiana) (P <0.05). After injection with 5 μg/μL of 20E dilution, the activity of PO and lysozyme in the black larvae of M. separata was significantly higher than that in the control (injecting anhydrous ethanol after being infected by B. bassiana ) at 8 h, 24 h after being infected by B. bassiana ( P <0.05). The experimental results suggest that MH plays an important role in regulating the humoral immune response of the black M. separata larvae. |
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