| 尤佳琪1, 李国庆2*.拟康宁木霉T-51菌株发酵液对灰葡萄孢的抑菌活性[J].植物保护,2021,47(1):74-78. |
| 拟康宁木霉T-51菌株发酵液对灰葡萄孢的抑菌活性 |
| Antifungal activity of Trichoderma koningiopsis T-51 cultural filtrate against Botrytis cinerea |
| 投稿时间：2019-08-25 修订日期：2019-11-11 |
| DOI：DOI:10.16688/j.zwbh.2019560 |
| 中文关键词: 拟康宁木霉T-51 灰葡萄孢 生物防治 抗真菌活性 |
| 英文关键词:Trichoderma koningiopsis T-51 Botrytis cinerea biological control antifungal activity |
| 基金项目:公益性行业(农业)科研专项(201303025) |
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| 中文摘要: |
| 拟康宁木霉菌株T-51是从土壤中分离得到的一株优秀生防菌株, 在前期的研究与应用中表现出对番茄灰霉病的良好防效?本文研究了T-51菌株的PDB发酵液对灰葡萄孢的抑菌活性, 结果表明T-51发酵液在0.5%的低浓度下的抑菌率达到52.1%, 与高浓度(10%)发酵液的抑菌率(59.7%)没有显著差异?当发酵液浓度下降为0.05%时失去抑菌活性?T-51摇培至第5天时的发酵液的抑菌活性稳定?T-51发酵液中的抑菌物质对高温不敏感, 沸水浴处理10 min后抑菌活性没有明显降低?T-51发酵液pH为3.3, 呈酸性, 当调节pH至7时, 发酵液抑菌活性从57.89%下降到46.61%?T-51发酵液能够显著抑制灰葡萄孢侵染番茄叶片后产生的病斑大小, 接种后叶片病斑直径比对照减小53.9%? |
| 英文摘要: |
| Trichoderma koningiopsis T-51, a promising biological control agent against gray mold caused by Botrytis cinerea, was isolated from soil. The antifungal activity of the cultural filtrates (CF) of isolate T-51 in potato dextrose broth (PDB) was determined. The results showed that 0.5% of T-51 CF showed high antifungal activity against B. cinerea, with an inhibition percentage of 52.1%, not significantly (P>0.05) different from that in the treatment with 10% CF (59.7%). The T-51 CF lost the antifungal activity at the concentration of 0.05%. The antifungal activity of the T-51 CF did not continue increase when incubated for more than 5 days. The antifungal metabolites of T-51 appeared thermostable, as the CF in the treatment at 100℃ for 10 min still showed antifungal activity. Ambient pH value greatly affected the antifungal activity of the T-51 CF: when the pH value of the CF was adjusted from 3.3 to 7.0 with NaOH, the inhibition percentage was decreased from 57.89% to 46.6%. The CF of T-51 showed strong inhibition against infection by B. cinerea on tomato leaves, the size of the average leaf lesion was decreased by 53.9% by the T-51 CF compared to that in the control. |
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