| 景亮亮, 姜 灵, 谢 婷, 张晓霞, 洪 波, 贾彦霞*.不同培养基继代培养蜡蚧轮枝菌对产孢量和烟粉虱毒力的影响[J].植物保护,2020,46(5):70-76. |
| 不同培养基继代培养蜡蚧轮枝菌对产孢量和烟粉虱毒力的影响 |
| Influences of subculture on the virulence to Bemisia tabaci and conidial production of the entomopathogenic fungus Verticillium lecanii |
| 投稿时间：2019-07-05 修订日期：2019-09-18 |
| DOI：DOI: 10.16688/j.zwbh.2019343 |
| 中文关键词: 烟粉虱 蜡蚧轮枝菌 继代培养 毒力 产孢量 |
| 英文关键词:Bemisia tabaci Verticillium lecanii subculture virulence conidial production |
| 基金项目:宁夏“十三五”重点研发计划重大项目（2018BBF02021-02）；宁夏自然科学基金（2018AAC03038） |
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| 中文摘要: |
| 为了探究不同培养基继代培养的蜡蚧轮枝菌对烟粉虱的作用效果, 通过继代培养方法测定JMC-01、JMC-02菌株的产孢量、对烟粉虱的毒力及其几丁质酶活性。结果表明, JMC-01、JMC-02菌株在添加蝉蜕和烟粉虱的PDA培养基中连续培养5代的产孢量、对烟粉虱的毒力、几丁质酶活性均高于无添加的PDA培养基, 且JMC-01菌株的各项参数均高于JMC-02菌株。JMC-01菌株在添加蝉蜕的PDA培养基中T1和T3代的产孢量最大, 均为1.279×107个/mL, T3代对烟粉虱的毒力最大, 为88.33%, T5代的几丁质酶活最高, 为0.268 3 U/mL; 在添加烟粉虱的PDA培养基中, JMC-01菌株的最大产孢量、对烟粉虱的最高毒力和最高几丁质酶活分别出现在S2、S4和S5代, 分别为1.270×107个/mL、88.33%和0.325 4 U/mL。回归分析结果显示, JMC-01、JMC-02菌株几丁质酶活性与烟粉虱校正死亡率呈显著的正相关, 且拟合优度较好。本试验表明JMC-01、JMC-02菌株均对烟粉虱具有较强的毒力, 添加蝉蜕和烟粉虱的培养基可以维持菌株生长特性、增加产孢量、延缓菌株退化、增强菌株的几丁质酶活性, 为烟粉虱的生物防治提供了新的菌种资源。 |
| 英文摘要: |
| In order to explore the effects of Verticillium lecanii subculture on different culture media on Bemisia tabaci, the sporulation of JMC-01 and JMC-02 strains, their virulence to B. tabaci and the chitinase activity were determined by using subculture method. The results showed that the conidial production, the virulence to B. tabaci and chitinase activity of JMC-01 and JMC-02 strains subcultured for 5 generations in the PDA medium supplemented with cicada slough and B. tabaci corpses were higher than those without supplements. The various parameters of JMC-01 strain were higher than those of JMC-02 strain. The JMC-01 strain had the maximum sporulation in the PDA medium supplemented with cicada slough in T1 and T3 generations, both of which were 1.279×107 spores/mL. The T3 generation had the highest virulence to B. tabaci, which was 88.33%. The highest chitinase activity was 0.268 3 U/mL in T5 generation. The maximum sporulation, the highest virulence to B. tabaci and the highest chitinase activity of JMC-01 strain in the PDA medium supplemented with B. tabaci corpses were observed in S2, S4 and S5 generations, which were 1.270×107 spores/mL, 88.33% and 0.325 4 U/mL, respectively. The results of regression analysis showed that the chitinase activity of the two strains was significantly positively correlated with the corrected mortality of B. tabaci with a high goodness of fit. This study showed that both JMC-01 and JMC-02 strains had strong virulence to B. tabaci, and the media containing cicada slough and B. tabaci corpses could maintain the growth characteristics of the strains, increased the sporulation, delayed the degradation of the strains, and enhanced the chitinase activity of the strains, which provides new fungal resources for the biological control of B. tabaci. |
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