| 曹学仁1, 周益林2*.基于PCR技术的植物病原菌分子定量检测技术研究进展[J].植物保护,2020,46(4):7-11. |
| 基于PCR技术的植物病原菌分子定量检测技术研究进展 |
| Research progress in quantitative detection of plant pathogens using PCR technique |
| 投稿时间：2019-09-05 修订日期：2019-10-11 |
| DOI：10.16688/j.zwbh.2019463 |
| 中文关键词: 植物病原菌 定量检测 实时定量PCR 数字PCR 活体检测 |
| 英文关键词:plant pathogen quantitative detection real-time quantitative PCR digital PCR |
| 基金项目:国家自然科学基金（31701731, 31972212）；中国热带农业科学院基本科研业务费专项（1630042017003） |
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| 中文摘要: |
| 植物病原菌的菌源量是病害发生和流行的重要因子之一,对其精准的定量测定或检测可大大提高植物病害预测的准确性,本文对实时荧光定量PCR (qPCR)与数字PCR在植物病原菌定量检测、以及基于RNA水平的real-time PCR和基于核酸染料(EMA/PMA)与qPCR相结合的技术在植物病原菌活体定量检测中的应用进行了综述,并展望其在植物病害流行和预测中的应用前景。 |
| 英文摘要: |
| The inoculum of plant pathogens is an important factor related with disease epidemics. Precise quantification of inoculum greatly helps the prediction of diseases. This review summarized the applications of real-time quantitative PCR (qPCR) and digital PCR in the quantification of plant pathogens. The use of real-time reverse-transcriptase PCR and the combination of viability dyes and qPCR in viability detection of plant pathogens were also reviewed. The future perspectives of molecular quantitative detection of the pathogens in plant disease epidemics were discussed. |
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