傅 昱#, 李燕霞#, 刘婉蓉, 章 攀, 游春平*.岗梅枝枯病病原鉴定[J].植物保护,2019,45(3):170-173. |
岗梅枝枯病病原鉴定 |
Identification of the pathogen causing branch blight of Ilex asprella |
投稿时间:2018-06-22 修订日期:2018-07-24 |
DOI:10.16688/j.zwbh.2018268 |
中文关键词: 岗梅 枝枯病 病原鉴定 小孢拟盘多毛孢菌 |
英文关键词:Ilex asprella branch blight identification of the pathogen Pestalotiopsis microspora |
基金项目:广东省现代农业花卉产业技术体系(2017LM1131) |
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中文摘要: |
为明确引起岗梅Ilex asprella枝枯病的病原, 对从广东梅州采集的岗梅枝枯病病部分离的致病菌株Gangmei 2进行了形态学观察, 并运用MEGA X构建了病原菌株rDNA内转录间隔区(internal transcribed spacer, ITS)的系统发育树。结果显示:病原菌的形态学特征与拟盘多毛孢属Pestalotiopsis真菌相似, 在系统发育树中该菌株与小孢拟盘多毛孢菌Pestalotiopsis microspora在同一分支上。因此, 初步将岗梅枝枯病病原菌鉴定为小孢拟盘多毛孢菌P. microspora。 |
英文摘要: |
The pathogen causing branch blight of Ilex asprella was identified by Koch’s postulates, morphological characteristics and phylogeny analysis of rDNA internal transcribed spacer (ITS). The results showed that the pathogenic isolate Gangmei 2 from diseased I.asprella samples from Meizhou, Guangdong province was similar to Pestalotiopsis fungus in morphological characteristics. The isolate Gangmei 2 was in the same clade with Pestalotiopsis microspora by construction of phylogenetic tree between the isolate and the related isolates from GenBank.Therefore, the pathogen that causing branch blight of I.asprella was preliminarily identified as P.microspora. |
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