| 陈 琦1, 侯艳红1, 段 云2, 范志业1, 沈海龙1, 陈 莉1, 刘 迪1, 师兴凯1, 李世民1*.黏虫蛹性别的快速鉴别方法[J].植物保护,2019,45(2):157-159. |
| 黏虫蛹性别的快速鉴别方法 |
| Rapid sex identification of Mythimna separata pupae |
| 投稿时间：2018-03-22 修订日期：2018-04-25 |
| DOI：10.16688/j.zwbh.2018127 |
| 中文关键词: 黏虫 蛹 性别鉴别 形态学特征 |
| 英文关键词:Mythimna separata pupae sex identification morphological character |
| 基金项目:国家公益性行业(农业)科研专项(201403031)；河南省现代农业产业技术体系建设专项(Z2015-01-06) |
| 作者 | 单位 | | 陈 琦1, 侯艳红1, 段 云2, 范志业1, 沈海龙1, 陈 莉1, 刘 迪1, 师兴凯1, 李世民1* | 1.河南省漯河市农业科学院, 漯河 462300
2.河南省农业科学院植物保护研究所, 郑州 450002 |
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| 中文摘要: |
| 根据黏虫Mythimna separata(Walker)蛹生殖孔的位置、腹部腹节的外部形态学特征、生殖孔(产卵孔)至排泄孔之间的距离, 快速、准确鉴别黏虫蛹性别。雌蛹第8腹节腹面中央的生殖孔与第9腹节的产卵孔连接形成一纵裂缝, 裂缝两侧平坦, 无突起。生殖孔下端与第9腹节的前缘连接、产卵孔下端与第10腹节的前缘连接, 形成2个明显的“人”字纹。雄蛹第8腹节无纵裂缝, 在第9腹节腹面中央的生殖孔呈现的纵裂缝连接第10腹节, 裂缝两边各有一个半圆形瘤状突起。雌雄蛹排泄孔均在第10腹节腹面, 雌蛹产卵孔下端至排泄孔上端之间的距离为(400.85±151.10)μm, 雄蛹生殖孔下端至排泄孔上端之间的距离为(178.21±93.59)μm, 约为雌蛹相应距离的一半。采用该方法观察的270头羽化蛹的性别鉴别准确率100%。该方法对于快速鉴别黏虫蛹的性别、开展黏虫相关的生物学研究以及预测预报等具有重要的参考价值。 |
| 英文摘要: |
| Based on the position of gonopore, the morphology of the 8th, 9th and 10th abdominal segments, and the distance between gonopore or oviposition hole and anus, a method for rapid and accurate sex identification of Mythimna separata (Walker) pupae was described. The female pupa has a longitudinal crack from gonopore in the middle of the 8th abdominal segments to oviposition hole in 9th abdominal segments. Both sides of the crack are flat and without humps. The bottom of the gonopore was connected with the front of the 9th abdominal segment, and the bottom of the oviposition pore was connected with the front of the 10th abdominal segment, forming two obvious herringbone patterns. The male pupa has no crack on the 8th abdominal segment, but a longitudinal gonopore crack from the middle of 9th abdominal segment to the margin of the 10th abdominal segment. There is a visible lump on both sides of the crack, respectively. The anus of female and male are all on the 10th abdominal segment. The distance from the margin of the oviposition hole to the anus in the female pupae (400.85 μm±151.10 μm) is two times longer of that from the gonopore to the anus in the male (178.21 μm±93.59 μm). The sex of observed 270 pupae was identified accurately with this method. The method is useful for rapid sex identification of M. separata pupae, biological research on M. separata and prediction of population dynamics. |
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