李树江, 周雪林, 杨友联*.太子参叶斑病病原菌鉴定及防治药剂筛选[J].植物保护,2018,44(3):182-185. |
太子参叶斑病病原菌鉴定及防治药剂筛选 |
Pathogen identification of leaf spot on Pseudostellaria heterophylla and screening of fungicides for its control |
投稿时间:2017-08-02 修订日期:2017-09-30 |
DOI:10.16688/j.zwbh.2017284 |
中文关键词: 太子参 Ascochyta versabilis 分子系统学 药剂筛选 |
英文关键词:Pseudostellaria heterophylla Ascochyta versabilis molecular phylogeny screening of fungicides |
基金项目:科技部科技基础性工作专项(2014FY120100); 贵州省特色重点实验室建设项目(黔教合KY字[2017]012); 六盘水市科技局科技合作项目(52020-2012-01-09-05); 贵州省教育厅重点学科植物学支持项目(2014); 六盘水师范学院科技创新团队项目(LPSSYKJTD201602) |
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中文摘要: |
采用形态学结合分子系统学方法对引起贵州黄平县和六枝道地中药材种植基地太子参叶斑病的病原菌进行鉴定, 并以菌丝生长法对75%百菌清可湿性粉剂、70%甲基硫菌灵可湿性粉剂、37%苯醚甲环唑可湿性粉剂、40%氟硅唑乳油以及1%申嗪霉素悬浮剂进行了室内药效筛选。结果表明:引起贵州太子参叶斑病的病原菌为 Ascochyta versabilis, 该病原菌为太子参病害新记录; 5种杀菌剂除75%百菌清可湿性粉剂2 000倍对 A.versabilis 的抑菌率较低外(51.4%), 其余4种供试杀菌剂均具有较强的抑菌作用。70%甲基硫菌灵可湿性粉剂2 000倍、37%苯醚甲环唑可湿性粉剂2 000倍和40%氟硅唑乳油2 000倍对 A.versabilis 的生长具有较强的抑制作用, 其抑制率均高于90%; 1%申嗪霉素悬浮剂2 000倍对该病原菌的抑制率为72.3%。 |
英文摘要: |
Based on morphological characteristics and ITS phylogeny analysis, the pathogen caused leaf spot on Pseudostellaria heterophylla was isolated and identified from Liuzhi and Huangping, Guizhou Province. The inhibition effects of chlorothalonil 75% WP, thiophanate-methyl 70% WP, difenoconazole 37% WP, flusilazole 40% EC, phenazino-1-carboxylic acid 1% SC on the pathogen of leaf spot were tested by mycelial growth method in laboratory. The results indicated that the pathogen was identified to be Ascochyta versabilis. Among the five fungicides, chlorothalonil 75% WP (2 000×) showed lower inhibition rates (51.4%) against A.versabilis, while the other four fungicides had good control efficacies, with the inhibition rates of more than 90% for difenoconazole 37% WP (2 000×), thiophanate-methyl 70% WP (2 000×) and flusilazole 40% EC (2 000×), and 72.3% for phenazino-1-carboxylic acid 1% SC (2 000×), respectively. |
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