| 罗欢, 贾国庚, 范小燕, 刘海峰, 裴东方, 邓建新*, 周燚*.一种波斯菊叶枯病新病原菌的分离与鉴定[J].植物保护,2017,43(6):182-186. |
| 一种波斯菊叶枯病新病原菌的分离与鉴定 |
| Isolation and identification of a new pathogen causing leaf blight on Cosmos bipinnatus |
| 投稿时间：2017-06-28 修订日期：2017-07-24 |
| DOI： |
| 中文关键词: 侵染向日葵链格孢 波斯菊 叶枯病 形态学 多基因序列 |
| 英文关键词:Alternaria helianthiinficiens Cosmos bipinnatus leaf blight morphology sequence analysis |
| 基金项目:国家自然科学基金(31400014); 长江大学青年人才基金(2016cqr08) |
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| 中文摘要: |
| 波斯菊是一种重要的观赏植物,叶枯病的发生影响其生长及市场价值。本文从陕西杨凌地区采集波斯菊病叶，通过保湿培养、单孢分离方法获得2株分离物。将分离菌的纯培养物接种健康寄主植物，发病后再分离的分离物与原接种病原菌菌株特征相同。采用形态学及rDNA-ITS、EF-1α和GAPDH基因序列分析相结合的方法对分离菌进行鉴定，结果表明，两株菌株均为侵染向日葵链格孢Alternaria helianthiinficiens。这是国内外波斯菊上A.helianthiinficiens的首次报道。 |
| 英文摘要: |
| Cosmos bipinnatus is an important ornamental plant. The leaf blight disease can affect plant growth and market value. In this study, the causal agent was obtained from diseased leaves by single spore isolation and its pathogenicity was tested to fulfill the Koch’s rule. Based on the morphological characteristics and phylogenetic analysis of a combined gene sequence dataset (rDNA-ITS, EF-1α and GAPDH), the pathogen was identified as Alternaria helianthiinficiens. It’s the first report of leaf blight disease on C. bipinnatus caused by A. helianthiinficiens in the world. |
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