| 龙海波1*, 陈文2, 裴月令1, 冯推紫1, 孙燕芳1.贵州省大豆孢囊线虫的发生与分布[J].植物保护,2017,43(6):173-176. |
| 贵州省大豆孢囊线虫的发生与分布 |
| Occurrence and distribution of Heterodera glycines in Guizhou Province |
| 投稿时间：2017-04-30 修订日期：2017-05-28 |
| DOI： |
| 中文关键词: 贵州省 大豆孢囊线虫 发生 分布 |
| 英文关键词:Guizhou Province Heterodera glycines occurrence distribution |
| 基金项目:国家公益性行业(农业)科研专项(201503114); 中国热带农业科学院基本科研业务费专项资金(1630042017024) |
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| 中文摘要: |
| 大豆孢囊线虫病是威胁大豆生产的重大病害之一。2015-2016年,采用随机取样的方法对贵州省大豆孢囊线虫的发生和分布情况进行了调查。调查范围包括贵阳、六盘水、安顺、毕节、黔西南和遵义等6个地区下辖的14个县(区),共采集土样287份。调查结果表明,贵州省大豆孢囊线虫的总检出率为28.2%,其中毕节地区的大方县和赫章县孢囊检出率最高,分别达到46.7%和45.0%,而在遵义市、贵阳市和黔西南州孢囊检出率为0。六盘水地区的水城县孢囊发生量最大,平均孢囊数为3.4个/200 g土,最大孢囊数达到17个/200 g土; 安顺地区的镇宁和关岭县大豆孢囊线虫发生程度最轻,每200 g土样平均孢囊数分别为0.3和0.2个,最大孢囊数仅为2个/200 g土。分离的孢囊主要形态特征与大豆孢囊线虫一致,并且ITS序列与甘肃大豆孢囊线虫种群序列一致性达100%。根据形态学观察和ITS序列分析比对,将贵州省采集到的孢囊线虫鉴定为大豆孢囊线虫。本研究结果可为有效防控贵州省大豆孢囊线虫病提供依据。 |
| 英文摘要: |
| Soybean cyst nematode (Heterodera glycines) is the most devastating and yield limiting pest of soybean in the world. A systematic survey of H.glycines in Guizhou Province was conducted during 2015-2016. A total of 287 soil samples were collected covering 14 counties from Guiyang, Liupanshui, Anshun, Bijie, Qianxinan and Zunyi. The results showed that the total incidence of soybean cyst nematode was 28.2%. Dafang and Hezhang showed the highest incidence of 46.7% and 45.0%, respectively. No cyst was found in Zunyi, Guiyang and Qianxinan. The highest cyst number existed in Shuicheng County, with the average density of 3.4 cysts per 200 g soil, and the max number of cysts was 17 per 200 g soil. The lowest cyst number existed in Zhenning and Guanling, with the average density of 0.3 and 0.2 cysts per 200 g soil, respectively, and both max number of cysts was only 2 per 200 g soil. Morphology of the cysts was the same as that for H.glycines, and the ITS sequence had 100% identity with H.glycines Gansu isolate in GenBank. Therefore, the cyst nematodes were identified as Heterodera glycines based on morphological characteristics and ITS sequences.This result can provide a basis for effective prevention of soybean cyst nematode in Guizhou Province. |
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