| 宋晓兵1, 2, 彭埃天1*, 程保平1, 凌金锋1, 陈 霞1, 张炼辉2.一株侵染柑橘木虱的球孢白僵菌的分离及鉴定[J].植物保护,2017,43(4):139-144. |
| 一株侵染柑橘木虱的球孢白僵菌的分离及鉴定 |
| Isolation and identification of a Beauveria bassiana strain infecting Diaphorina citri |
| 投稿时间：2016-04-11 修订日期：2016-07-16 |
| DOI： |
| 中文关键词: 柑橘木虱 球孢白僵菌 致病性 生物防治 |
| 英文关键词:Diaphorina citri Beauveria bassiana pathogenicity biocontrol |
| 基金项目:广东省农业领域引导项目(2013B020309004)；广东省科技计划项目(广东省农作物病虫害绿色防控技术研究开发中心建设)；广东省公益研究与能力建设项目(2014B020203003)；广东省现代农业产业技术体系建设专项(2016LM1077) |
| 作者 | 单位 | | 宋晓兵1, 2, 彭埃天1*, 程保平1, 凌金锋1, 陈 霞1, 张炼辉2 | 1. 广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640
2. 华南农业大学农学院, 广东省微生物信号与作物病害防控重点实验室, 广州 510640 |
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| 中文摘要: |
| 从柑橘木虱虫体分离得到一株具有强致病性的虫生真菌GZMS-28, 本文测定了该菌株对柑橘木虱的致病性, 并分析了其rDNA-ITS基因序列。试验结果表明, 该菌株对柑橘木虱具有较强的致病性, 浓度为1.0×108 个/mL的分生孢子悬浮液处理柑橘木虱成虫7 d后, 其校正死亡率达到95.7%。菌株GZMS-28的培养特征与白僵菌属球孢白僵菌较为一致, 其rDNA-ITS序列与GenBank中白僵菌属多个球孢白僵菌菌株的对应序列相似性达到99%以上, 因此将菌株GZMS-28鉴定为球孢白僵菌Beauveria bassiana。 |
| 英文摘要: |
| The strain GZMS-28 with strong pathogenicity was isolated from Diaphorina citri and its pathogenicity against D. citri was examined. Its rDNA-ITS gene sequence was also analyzed. D. citri adults were treated by conidial suspension at the concentration of 1.0×108 spores/mL, and the corrected mortality rate was 95.7% after 7 days. The results showed that the strain has a strong pathogenicity against D. citri. The study found that the morphological characteristics of the strain were consistent with that of Beauveria bassiana. The sequence alignment result showed that the strain GZMS-28 shared 99% homology with the sequences retrieved from GenBank (acc. nos. JX122736, JQ320365, GU565572 and AY531972). According to the morphological characteristics and rDNA-ITS sequence analysis, the strain GZMS-28 was identified as B. bassiana |
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