严婉荣, 肖 敏, 赵志祥, 陈 圆, 符尚娇, 肖彤斌*.辣椒细菌性叶斑病病原鉴定及室内药剂筛选[J].植物保护,2016,42(5):205-209. |
辣椒细菌性叶斑病病原鉴定及室内药剂筛选 |
Pathogen identification and bactericide screening of pepper bacterial leaf spot |
投稿时间:2015-09-17 修订日期:2015-12-15 |
DOI: |
中文关键词: 辣椒细菌性叶斑病; 病原菌 杀菌剂 药剂筛选 |
英文关键词:pepper bacterial leaf spot pathogenic bacteria bactericide bactericide screening |
基金项目:海南省科学事业费项目(KYYS-2014-36);“十二五”国家科技支撑计划(2012BAD19B06) |
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中文摘要: |
2012-2015年,在海南省辣椒主产区发生了一种严重危害辣椒的叶斑病,从发病叶片中分离到一种细菌,通过致病性测定、柯赫氏法则验证及分子生物学鉴定,将引起该病害的病原鉴定为野油菜黄单胞菌辣椒斑点病致病变种(Xanthomonas campestris pv. vesicatoria)。选用8种杀菌剂通过平板对峙法对该病原进行了室内毒力测定,结果表明:其中6种药剂对该病原有抑菌效果,效果最好的为72%农用硫酸链霉素可湿性粉剂,47%春雷·王铜可湿性粉剂次之,2%春雷霉素水剂和20%噻菌铜悬浮剂没有抑菌效果,不同药剂之间或同一种药剂不同剂型之间抑菌效果差异较大。病原鉴定和药剂筛选可为该病害的诊断和防治提供科学依据。 |
英文摘要: |
In 2012-2015, a serious pepper bacterial leaf spot disease occurred in the main producing areas of Hainan Province. A bacterial strain was isolated from the diseased pepper leaves, and identified as Xanthomonas campestris pv. vesicatoria by pathogenicity test, Koch’s postulates and molecular identification. Toxicities of 8 kinds of bactericide were tested by plate confrontation method. The results showed that 6 agents have antibacterial effects, and 72% streptomycin WP was most effective, and followed by 47% kasugamycin· copper oxychloride WP,while 2% kasugamycin AS and 20% thiodiazole copper SC have no inhibitory action. Antibacterial activity differed significantly between the different agents, and the same agents with different formulation also had different antibacterial effects |
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