何琼1, 吴海燕1*, 王振华2.山东泰安春秋季节大棚番茄根内根结线虫的发育及动态[J].植物保护,2016,42(4):184-188. |
山东泰安春秋季节大棚番茄根内根结线虫的发育及动态 |
Development and dynamics of root-knot nematode in tomato rootunder greenhouse in spring and autumn season |
投稿时间:2015-08-21 修订日期:2015-10-10 |
DOI: |
中文关键词: 根结线虫 温度 发育 动态 番茄 |
英文关键词:root-knot nematode temperature development dynamics tomato |
基金项目:公益性行业(农业)科研专项(201503114);国家自然科学基金(31460464);广西自然科学基金(2014GXNSFAA118123) |
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中文摘要: |
于2008年秋季和2009年春季对‘毛粉’和‘金丰盛世’2个番茄品种采用网袋法收集根系, 酸性品红-次氯酸钠法染色, 体视显微镜下检测, 记录根内各龄期根结线虫数量和形成的根结数。结果表明, 单位鲜根重线虫数量在2008年温度低时, 随番茄生长有下降趋势, 2009年春季温度升高时则上升, 2个供试品种单位鲜根重线虫数无显著差异(P>0.05); 2008年秋季J2到J3, J3到J4的发育时间均长于2009年, 根部形成根结的数量变化趋势相同; 当土壤平均温度为15℃时, 根结线虫在番茄根内完成第1个世代需32 d, 25℃时为24 d。 |
英文摘要: |
The tomato roots of cultivar ‘Maofen’ and ‘Jinfengshengshi’ were collected with mesh bag method in 2008 autumn and 2009 spring, the nematodes in root were stained by the method of acid fuchsin-NaClO, and the number of nematodes (including juveniles at different stage) and root galls were recorded using stereomicroscope. The results in two years indicated that the number of nematodes per gram fresh root was decreased with the growth of tomato under lower temperature in 2008 and increased under higher temperature in 2009. There was no significant difference in the number of nematodes per gram fresh root between two tested cultivars (P>0.05). The development time from J2 to J3 and J3 to J4 was longer in 2008 than that in 2009. The number of root galls in 2008 and 2009 showed the similar curve within 48 days after transplanting. Under this experimental condition, at the average soil temperature of 15℃, the first generation of root-knot nematode lasted for 32 days after transplanting, and for 24 days at the average soil temperature of 25℃. |
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