邓业成;李洁荣;高成伟;杨林林.广西地不容提取物及其化合物的抑菌活性[J].植物保护,2006,32(4):43-46. |
广西地不容提取物及其化合物的抑菌活性 |
Inhibitory activity of the extract from the root tubers of Stephania kwangsiensis and its compounds against pathogenic fungi and bacteria |
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DOI: |
中文关键词: 广西地不容提取物 病原菌 抑菌活性 |
英文关键词:extracts of Stephania kwangsiensis Lo. pathogenic fungi and bacteria inhibitory activities |
基金项目: |
邓业成;李洁荣;高成伟;杨林林 |
广西师范大学生命科学学院 桂林541004 |
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中文摘要: |
在室内用生长速率法测定了广西地不容块根提取物及其化合物对梨褐斑病菌、梨黑斑病菌、柑橘疮痂病菌和柑橘溃疡病菌的抑菌活性。结果表明,广西地不容块根提取物对上述4种病原菌均有明显的抑制作用,质量浓度为10 g/L时,72 h的抑菌率分别为100%、91.96%、84.76%和100%;在从广西地不容块根提取物分离出的7个化合物中,l-罗默碱对4种病菌均有很高的抑菌活性,质量浓度为1 g/L时,72 h的抑菌率分别为100%、100%、85.04%和100%。紫堇定对梨黑斑病菌抑菌活性高,72 h抑菌率为100%,对梨褐斑病菌和柑橘疮痂病菌也有较高的抑菌活性,而对柑橘溃疡病菌的抑菌活性低。广西地不容块根提取物对梨褐斑病菌、梨黑斑病菌、柑橘疮痂病菌和柑橘溃疡病菌的EC50分别为1.252 5、2.379 3、1.758 2、1.510 0 g/L,l-罗默碱的EC50分别为0.147 3、0.167 1、0.3464、0.118 2 g/L。 |
英文摘要: |
The inhibitory activity of the extract from the root tubers of Stephania kwangsiensis Lo.and its compounds against Mycosphaerella sentina Schroter,Alternaria kiknchiana Tanaka,Sphaceloma fawcetti Jenk.and Xanthomonas citri(Hasse.) Dowson was determined in laboratory using the growth rate method.The results showed that the extract from the root tubers of S.kwangsiensis had a significant inhibitory activity against the four pathogenic fungi and bacteria,and the inhibitory rates of the extract at a concentration of 10 g/L were 100%,(91.96%,) 84.76% and 100% after 72 h of treatment,respectively.As for the seven compounds isolated from the root tubers of S.kwangsiensis ,l-roemerine had high inhibitory activity against the four pathogenic fungi and bacteria,and the inhibitory rates of l-roemerine at a concentration of 1 g/L were 100%,100%,85.04% and 100% after 72 h of treatment,respectively,while corydine had high inhibitory activity against A.kiknchiana, medium inhibitory activity against M.sentina and S.fawcetti [WTBZ],low inhibitory activity against X.citri and 100% inhibitory rate against A.kiknchiana.The EC50 of the extract from the root tubers of S.kwangsiensis to M.sentina,A.kiknchiana,S.fawcetti and X.citri were 1.252 5,2.379 3,1.758 2 g/L and 1.510 0 g/L,respectively,whereas the EC50 of l-roemerine to the four pathogenic fungi and bacteria were 0.147 3,0.167 1,0.346 4 g/L and(0.118 2 g/L,) respectively. |
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