| 李宇晨, 马丽君, 张方正, 江 峰, 龚 成, 刘航玮, 罗 晨*.烟粉虱BtabHDAC3基因的鉴定及其在杀虫剂抗性中的功能分析[J].植物保护,2026,52(4):341-347. |
| 烟粉虱BtabHDAC3基因的鉴定及其在杀虫剂抗性中的功能分析 |
| Identification of the BtabHDAC3 in Bemisia tabaci and its functional analysis in insecticide resistance |
| 投稿时间：2025-09-17 修订日期：2025-11-05 |
| DOI：10.16688/j.zwbh.2025477 |
| 中文关键词: 烟粉虱 组蛋白去乙酰化酶 双丙环虫酯 抗药性 表观遗传调控 |
| 英文关键词:Bemisia tabaci histone deacetylase afidopyropen insecticide resistance epigenetic regulation |
| 基金项目:国家自然科学基金(32272522);国家资助博士后研究人员计划(GZC20241438) |
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| 中文摘要: |
| 组蛋白去乙酰化酶(HDACs)是调控昆虫生长发育、衰老等生命过程的关键表观遗传因子。HDAC3作为该家族核心成员, 在染色质重塑、细胞分化及代谢调控中扮演重要角色。本研究聚焦全球性重大害虫烟粉虱Bemisia tabaci, 成功克隆其BtabHDAC3基因(GenBank: PX289194)。该基因序列全长1 365 bp, 编码454个氨基酸;生物信息学分析表明, 其编码蛋白具有典型的组蛋白去乙酰化酶催化结构域和核定位信号, 且与其他昆虫HDAC3在保守基序上高度一致。系统进化分析显示, 烟粉虱BtabHDAC3与柑橘粉蚧Planococcus citri有较近的亲缘关系。通过RT-qPCR技术解析其时空表达模式, 发现该基因在卵期表达量最高, 且在成虫头部中特异性高表达, 暗示其在胚胎发育和神经功能中的潜在作用。进一步研究还发现烟粉虱雌虫BtabHDAC3在双丙环虫酯抗性种群的表达明显低于抗性衰减种群, 可能参与调控烟粉虱对杀虫剂的表观遗传抗性。本研究不仅为揭示烟粉虱表观遗传调控机制提供了关键分子靶点, 也为开发基于HDACs的新型害虫防控策略(如RNA农药或抗性逆转剂)奠定了坚实的理论基础。 |
| 英文摘要: |
| Histone deacetylases（HDACs）are crucial epigenetic regulators orchestrating various biological processes in insects, including development and aging. As a core member of the HDACs family, HDAC3 plays a pivotal role in chromatin remodeling, cell differentiation, and metabolic regulation. This study cloned BtabHDAC3 gene （GenBank: PX289194）from Bemisia tabaci, which spans 1 365 bp and encodes a 454-amino-acid protein. Bioinformatics analysis confirmed that the encoded protein possesses the classic histone deacetylase catalytic domain and a nuclear localization signal, showing high conservation in motifs with HDAC3s from other insect species. Phylogenetic analysis revealed the closest relationship of BtabHDAC3 with that of the Planococcus citri. Spatiotemporal expression profiling via RT-qPCR demonstrated that BtabHDAC3 expression was highest in the egg stage and specifically enriched in the head tissue of adults, suggesting its potential role in embryogenesis and neurological function. Most significantly, we discovered that BtabHDAC3 expression in females of an afidopyropen-resistant population was significantly lower than in a susceptible counterpart, indicating its potential involvement in mediating epigenetic resistance to insecticides. Our findings not only provide a key molecular target for understanding the epigenetic regulatory mechanisms in B.tabaci but also establish a solid theoretical foundation for developing novel HDACs-based pest control strategies, such as RNA pesticides or resistance reversal agents. |
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