| 徐晓玉 1, 李国强 2, 武 鑫 1, 李咏玲 1, 荆小院 2, 杨美红 1, 刘金龙 1*.六星黑点豹蠹蛾性信息素分泌腺的位置及外部形态[J].植物保护,2026,52(4):285-292. |
| 六星黑点豹蠹蛾性信息素分泌腺的位置及外部形态 |
| Location and morphology of the sex pheromone gland in Zeuzera leuconotum (Lepidoptera: Cossidae) |
| 投稿时间：2025-10-13 修订日期：2025-11-26 |
| DOI：10.16688/j.zwbh.2025522 |
| 中文关键词: 六星黑点豹蠹蛾 性信息素分泌腺 位置 形态 触角电位 扫描电镜 |
| 英文关键词:Zeuzera leuconotum sex pheromone gland location morphology electroantennography scanning electron microscopy |
| 基金项目:山西省基础研究计划项目(202103021224169);山西省自然科学基金(2010011042-2);山西农业大学博士科研启动项目(2016ZZ10) |
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| 中文摘要: |
| 昆虫性信息素分泌腺的位置及形态研究是阐述其化学通讯系统和性信息素化学成分鉴定的基础。研究表明, 大多数鳞翅目昆虫性信息素分泌腺位于其腹尖(第8～9腹节)节间膜上, 但六星黑点豹蠹蛾Zeuzera leuconotum Butler成虫自羽化后一直将腹尖暴露于体外, 与其他鳞翅目昆虫存在明显差异。为确定六星黑点豹蠹蛾性信息素分泌腺具体位置, 本研究对六星黑点豹蠹蛾雌虫不同部位提取物进行触角电位分析、风洞活性测定以及林间诱蛾试验。结果显示, 六星黑点豹蠹蛾腹尖提取物的电生理活性和诱蛾活性显著强于头、胸、足、翅、腹等部位, 表明其性信息素分泌腺位于腹尖。经光学显微镜和扫描电子显微镜观察, 在六星黑点豹蠹蛾第8～9腹节两侧节间膜有由特化的肥大表皮细胞组成的褶皱, 细胞呈单层排列, 表面分布着基部饱满的刺状体, 说明其分泌腺位于第8～9腹节节间膜的腹面两侧。六星黑点豹蠹蛾性信息素分泌腺位置和形态的阐明, 有助于研究其性信息素产生和释放的时辰节律, 对后续性信息素的提取、分离和鉴定具有重要意义。 |
| 英文摘要: |
| The location and morphology of insect sex pheromone glands are fundamental for elucidating chemical communication systems and identifying pheromone components. In most Lepidoptera, sex pheromone glands are located on the intersegmental membrane of the abdominal apex (segments 8-9). However, adults of Zeuzera leuconotum Butler continuously expose the abdominal apex after eclosion, which differs markedly from other Lepidoptera species. To determine the precise location of the sex pheromone gland in Z.leuconotum, electroantennography (EAG), wind tunnel bioassays, and field trapping experiments were conducted using extracts from different body parts of female moths. The results demonstrated that extracts from the abdominal apex exhibited significantly higher electrophysiological activity and attractiveness compared to those from the head, thorax, legs, wings, and abdomen, indicating that the pheromone gland is located at the abdominal apex. Light microscopy and scanning electron microscopy revealed that the intersegmental membranes on both sides of abdominal segments 8 and 9 contained folds composed of specialized hypertrophied epidermal cells. These cells were arranged in a single layer, and their surfaces were covered with spine-like structures with swollen bases. These observations further confirmed that the sex pheromone gland is located on the ventral intersegmental membranes between abdominal segments 8 and 9. Elucidation of the location and morphology of the sex pheromone gland in Z.leuconotum provides a basis for studying the temporal dynamics of pheromone biosynthesis and release, thereby facilitating subsequent studies on pheromone extraction, isolation, and identification. |
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