| 冯玲佳 1, 刘毓婕 1, 闫 会 1, 2, 陈思冶 1, 3, 何林彤 1, 王新超 1, 叶 萌 1*.芳樟醇对灰茶尺蠖生长发育及繁殖的抑制作用及其生理基础[J].植物保护,2026,52(4):272-284. |
| 芳樟醇对灰茶尺蠖生长发育及繁殖的抑制作用及其生理基础 |
| Inhibitory effect of linalool on growth, development and reproduction of Ectropis grisescens and the underlying physiological basis |
| 投稿时间：2025-10-21 修订日期：2025-12-20 |
| DOI：10.16688/j.zwbh.2025541 |
| 中文关键词: 灰茶尺蠖 芳樟醇 生长发育 繁殖 绿色防控 |
| 英文关键词:Ectropis grisescens linalool growth and development reproduction green control |
| 基金项目:浙江省自然科学基金(LR25C160002);国家自然科学基金(32372775);中国农业科学院青年英才计划引进工程(2021-2025) |
| 作者 | 单位 | E-mail | | 冯玲佳 1, 刘毓婕 1, 闫 会 1, 2, 陈思冶 1, 3, 何林彤 1, 王新超 1, 叶 萌 1* | 1. 中国农业科学院茶叶研究所, 国家茶树改良中心, 茶树种质创新与资源利用全国重点实验室, 农业农村部特种经济动植物生物学与遗传育种重点实验室, 杭州 310008
2. 华中农业大学, 武汉 430070
3. 贵州大学, 贵阳 550025 | yemeng@caas.cn |
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| 中文摘要: |
| 为探究植物源挥发物芳樟醇对灰茶尺蠖Ectropis grisescens低龄幼虫生长发育及成虫繁殖的抑制作用及其生理基础, 本研究采取饲喂和暴露两种方式以芳樟醇处理灰茶尺蠖幼虫, 分别从1龄和2龄起处理至化蛹, 系统测定两种处理对幼虫生长和成虫繁殖指标及其体内保护酶、解毒酶活性的影响。结果表明：幼虫自1龄和2龄起以芳樟醇饲喂处理, 幼虫期、蛹期和子代卵期显著延长, 成虫期、雌成虫寿命以及产卵历期显著缩短, 同时幼虫体重、化蛹率、蛹重、羽化率、单雌产卵量及子代卵孵化率显著降低, 且幼虫1龄起饲喂芳樟醇处理后幼虫存活率也显著降低。幼虫分别自1龄和2龄起经芳樟醇暴露处理后, 幼虫期显著延长, 且幼虫体重与单雌产卵量显著降低。幼虫1龄起暴露处理后, 蛹重、羽化率及子代卵孵化率也显著降低。2龄幼虫经暴露处理后蛹期显著延长。芳樟醇饲喂与暴露处理均可使1龄和2龄幼虫保护酶SOD及解毒酶CarE、AChE活性显著上升, 而保护酶CAT活性显著下降, 表明其引发氧化应激并扰乱了解毒代谢稳态。综上, 芳樟醇饲喂与暴露均可抑制灰茶尺蠖低龄幼虫的生长发育与繁殖, 其中饲喂处理作用更强, 且1龄幼虫敏感性更高。研究结果为芳樟醇作为植物源农药靶向灰茶尺蠖低龄幼虫防治提供理论依据。 |
| 英文摘要: |
| To investigate the inhibitory effects of the plant-derived volatile linalool on growth, development, and reproduction of early-instar larvae of Ectropis grisescens and its physiological mechanism, two treatment methods, feeding (dietary intake) and exposure (volatile contact), were adopted in this study. The 1st- and 2nd-instar larvae were continuously treated with linalool until pupation. The changes in larval growth and reproductive indices, together with the activities of protective enzymes and detoxification enzymes in larvae were systematically evaluated. The results showed that after the 1st- and 2nd-instar larvae were treated with linalool feeding, the developmental duration of larval, pupal, and offspring egg stages were significantly prolonged, while the adult stage, female adult longevity, and oviposition duration were significantly shortened. Meanwhile, larval body weight, pupation rate, pupal weight, eclosion rate, average fecundity per female, and offspring egg hatching rate all decreased significantly, and the survival rate of the 1st instar larvae was also notably reduced under feeding treatment. When the 1st- and 2nd-instar larvae were subjected to linalool exposure, their larval stage was significantly prolonged, and larval body weight as well as average fecundity per female were markedly reduced. For the 1st-instar exposure group, pupal weight, eclosion rate, and offspring egg hatching rate were also significantly decreased; for the 2nd-instar larvae exposed to linalool, the pupal stage was distinctly prolonged. Both linalool feeding and exposure treatments significantly increased the activities of protective enzyme superoxide dismutase (SOD) and detoxification enzymes including carboxylesterase (CarE) and acetylcholinesterase (AChE) in the 1st- and 2nd-instar larvae, whereas the activity of the protective enzyme catalase (CAT) was significantly inhibited, indicating that linalool triggered oxidative stress and disrupted the homeostasis of detoxification metabolic systems. In conclusion, linalool can interfere with the growth, development, and reproduction of early-instar larvae of E.grisescens through both feeding and exposure, with stronger inhibitory effects observed in feeding treatment, and the 1st-instar larvae exhibiting higher susceptibility. This study provides a theoretical basis for the application of linalool as a plant-derived pesticide in the targeted control of early-instar larvae of E.grisescens. |
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