| 郭含莹 1, 2#, 马艳华 2#, 董欢欢 2, 屈亚飞 2, 曹雅忠 2, 李克斌 2, PRIMITIVE Ishimwe Mukundwa 3, 张 烨 1*, 尹 姣 2*.根系分泌物对暗黑鳃金龟幼虫定位寄主植物根系的影响[J].植物保护,2026,52(4):146-154. |
| 根系分泌物对暗黑鳃金龟幼虫定位寄主植物根系的影响 |
| Influence of root exudates on host plant root localization in Holotrichia parallela larvae |
| 投稿时间：2025-11-24 修订日期：2025-12-23 |
| DOI：10.16688/j.zwbh.2025603 |
| 中文关键词: 暗黑鳃金龟幼虫 根系分泌物 电生理 趋性行为 吸引作用 |
| 英文关键词:Holotrichia parallela larvae root exudates electroantennography chemotactic behavior attractant effect |
| 基金项目:国家重点研发计划(2023YFE0125800) |
| 作者 | 单位 | E-mail | | 郭含莹 1, 2#, 马艳华 2#, 董欢欢 2, 屈亚飞 2, 曹雅忠 2, 李克斌 2, PRIMITIVE Ishimwe Mukundwa 3, 张 烨 1*, 尹 姣 2* | 1. 山西农业大学植物保护学院, 太原 030031
2. 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193
3. 卢旺达农业与动物资源发展局（RAB）, 基加利 5016, 卢旺达 | 张烨wuduhe@126.com;尹姣jyin@ippcaas.cn |
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| 中文摘要: |
| 为探究根系分泌物对暗黑鳃金龟 Holotrichia parallela 幼虫的引诱作用, 对其开展了触角和下颚须的电生理试验及幼虫的趋性行为试验。以空气为对照, 利用Y形嗅觉仪测定了 3 龄幼虫对马铃薯、玉米、花生和大豆根系的趋性反应;以液体石蜡为对照, 利用昆虫触角电位仪测定了 3 龄幼虫触角和下颚须对根系分泌物的电生理反应;并采用Y形嗅觉仪测定了 3 龄幼虫对 11 种根系分泌物的趋性行为反应。结果表明:幼虫对马铃薯、玉米、花生和大豆根系均有显著的趋向性;根系分泌物 2-丁烯酸、4-甲基-2-氧戊酸、正丁酸等能引起幼虫触角和下颚须较强烈的电生理反应;根系分泌物同样能引起幼虫的趋性行为反应, 2-丁烯酸(20、200、2 000 μg/mL)、4-甲基-2-氧戊酸(200、2 000 μg/mL)、对二甲苯(100、300、500、700 μg/mL)、肉豆蔻酸(80 μg/mL)、棕榈酸(2 000、2 200、2 400 μg/mL)、硬脂酸(1 000、1 200、1 400 μg/mL)、棕榈酸甘油酯(300、500、700 μg/mL)、甲基丁二酸(40、80、320 μg/mL)、苯乙烯(300、500、700 μg/mL)、十六烷(100、200、400、800 μg/mL)、正丁酸(200、2 000 μg/mL)对暗黑鳃金龟幼虫均具有显著的吸引作用。由以上结果可知, 寄主植物根系和根系分泌物可显著激发暗黑鳃金龟幼虫的趋向性行为反应或电生理活性, 推测暗黑鳃金龟幼虫通过识别根系分泌物实现对寄主植物根系的定位。 |
| 英文摘要: |
| To investigate the attractant effect of root exudates on Holotrichia parallela larvae, electrophysiological responses of the antennae and maxillary palps, as well as larval chemotactic behavior, were examined. Using air as the control, a Y-tube olfactometer was used to determine the chemotactic responses of the 3rd-instar larvae to the roots of potato, maize, peanut, and soybean. Using liquid paraffin as the control, electroantennography was employed to measure the electrophysiological responses of the antennae and maxillary palps of the 3rd-instar larvae to root exudates. Additionally, a Y-tube olfactometer was used to assess the chemotactic behavioral responses of the 3rd-instar larvae to eleven root exudates. The results showed that the larvae exhibited significant attraction to the roots of potato, maize, peanut, and soybean. Root exudates such as crotonic acid, 4-methyl-2-oxovaleric acid, and butyric acid induced strong electrophysiological responses in the antennae and maxillary palps. Root exudates also induced chemotactic behavioral responses in the larvae. Crotonic acid (20, 200, 2 000 μg/mL), 4-methyl-2-oxovaleric acid (200, 2 000 μg/mL), p-xylene (100, 300, 500, 700 μg/mL), myristic acid (80 μg/mL), palmitic acid (2 000, 2 200, 2 400 μg/mL), stearic acid (1 000, 1 200, 1 400 μg/mL), tripalmitin (300, 500, 700 μg/mL), 2-methylsuccinic acid (40, 80, 320 μg/mL), styrene (300, 500, 700 μg/mL), n-hexadecane (100, 200, 400, 800 μg/mL), and butyric acid (200, 2 000 μg/mL) all showed significant attractant effects on H.parallela larvae. These results indicate that host plant roots and their exudates can significantly induce chemotactic behavioral responses and electrophysiological activity in H.parallela larvae. It is inferred that H.parallela larvae locate host plant roots by recognizing root exudates. |
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