| 王若朴 1, 2, 谭 琳 2, 3, 任佐华 2, 3, 邓玉莲 1, 姚苏航 1, 胡秋龙 1, 2*.多产色链霉菌XS-4可湿性粉剂研制及其对茶树炭疽病的防治效果[J].植物保护,2026,52(4):131-139. |
| 多产色链霉菌XS-4可湿性粉剂研制及其对茶树炭疽病的防治效果 |
| Development of Streptomyces polychromogenes XS-4 wettable powder and its biocontrol efficacy on Colletotrichum coccodes |
| 投稿时间：2025-10-23 修订日期：2026-01-02 |
| DOI：10.16688/j.zwbh.2025544 |
| 中文关键词: 可湿性粉剂 多产色链霉菌XS-4 茶炭疽病 防治效果 |
| 英文关键词:wettable powder Streptomyces polychromogenes XS-4 tea anthracnose control efficacy |
| 基金项目:国家重点研发计划(2022YFD1600803);刘仲华院士创新团队云游茶叶工作室科研成果转化项目 |
| 作者 | 单位 | E-mail | | 王若朴 1, 2, 谭 琳 2, 3, 任佐华 2, 3, 邓玉莲 1, 姚苏航 1, 胡秋龙 1, 2* | 1. 湖南农业大学园艺学院, 长沙 410128
2. 岳麓山实验室, 长沙 410128
3. 湖南农业大学植物保护学院, 长沙 410128 | huqiulongnet@126.com |
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| 中文摘要: |
| 本研究以茶树炭疽病病原菌Colletotrichum coccodes为靶标, 将生防菌株多产色链霉菌Streptomyces polychromogenes XS-4研制成防治茶树炭疽病的微生物菌剂, 首先通过单因素试验优化发酵条件, 得到最佳发酵参数:以麦芽糖为碳源、胰蛋白胨为氮源, pH 6.0、200 mL发酵体系接种20 mL种子发酵液。在此基础上, 对载体与助剂配比进行了筛选和优化, 其最佳配方为:白炭黑为载体(母粉89%), 助剂10%(拉开粉与二丁基羟基甲苯质量比3∶7), 紫外保护剂1%(抗坏血酸), 在上述条件下得到的多产色链霉菌XS-4可湿性粉剂, 孢子含量达1.53×10^9 cfu/g, 悬浮率90.58%, 湿润时间41.75 s, pH 6, 100倍稀释液的田间试验防效达64.72%。本研究为茶树炭疽病的绿色防控提供了可行的微生物菌剂产品与技术支撑。 |
| 英文摘要: |
| This study aimed to develop a microbial biocontrol agent derived from Streptomyces polychromogenes XS-4 to control tea anthracnose caused by Colletotrichum coccodes. Through single-factor experiments, the optimal fermentation conditions were determined as follows: maltose as the carbon source, tryptone as the nitrogen source, pH 6.0, and an inoculum size of 20 mL. Subsequently, a wettable powder formulation was developed by optimizing the carrier and adjuvant ratios, with the final composition consisting of 89% master powder (using white carbon black as the carrier), 10% adjuvants (Nekal and butylated hydroxytoluene at a 3∶7 mass ratio), and 1% UV protectant (ascorbic acid). Quality evaluation demonstrated that the formulated product exhibited a spore content of 1.53×10 9 spores/g, a suspension rate of 90.58%, a wetting time of 41.75 s, and a pH value of 6. Field control efficacy of its 100-fold dilution reached 64.72%. This study provides an effective microbial agent and technical foundation for the eco-friendly control of tea anthracnose. |
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