| 王连春 1, 李学美 2, 乔 开 2, 赵 静 1, 虎思雨 1, 罗秋洁 1, 周海霞 1, 高 达 1, 王武雄 1, 马晔铭 3, 万琼莲 1*.云南玉溪不同地区山黄麻丛枝植原体分子鉴定及多位点基因分析[J].植物保护,2026,52(4):76-87. |
| 云南玉溪不同地区山黄麻丛枝植原体分子鉴定及多位点基因分析 |
| Molecular identification and multilocus gene analysis of Trema levigata witches’-broom phytoplasma from different regions of Yuxi, Yunnan |
| 投稿时间：2025-08-13 修订日期：2025-10-20 |
| DOI：10.16688/j.zwbh.2025423 |
| 中文关键词: 植原体 山黄麻 16SrⅠ-Ⅹ 丛枝病 多位点分析 |
| 英文关键词:phytoplasma Trema levigata 16SrⅠ-Ⅹ witches’-broom disease multilocus analysis |
| 基金项目:云南省地方本科高校基础研究联合专项-青年项目(202101BA070001-274);云南省地方本科高校基础研究联合专项-面上项目(202401BA070001-153) |
| 作者 | 单位 | E-mail | | 王连春 1, 李学美 2, 乔 开 2, 赵 静 1, 虎思雨 1, 罗秋洁 1, 周海霞 1, 高 达 1, 王武雄 1, 马晔铭 3, 万琼莲 1* | 1. 玉溪师范学院农学与生物科学学院, 玉溪 653100
2. 云南农业大学植物保护学院, 昆明 650201
3. 玉溪晔艺生物科技有限公司, 玉溪 653100 | wanqionglian@yxnu.edu.cn |
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| 中文摘要: |
| 近年来, 山黄麻丛枝病在云南不同地区不断被发现, 感病植株呈现丛枝、小叶症状, 受害严重。为确认该病害病原、病原分类地位及分离株之间的亲缘关系, 本研究通过PCR扩增16S rRNA 基因和rp, tuf, secA, secY, ipt, dnaK, fusA, gyrB, pyrG, rpoB 10个管家基因进行遗传进化分析, 并结合植原体在线工具 iPhyClassifier 对分离株进行分类鉴定。结果表明, 云南元江、易门、新平和峨山的山黄麻丛枝病病原都属于翠菊黄化植原体复合群（Candidatus Phytoplasma asteris）, 为植原体16SrⅠ-Ⅹ亚组成员。fusA、gyrB和rpoB基因系统进化树显示4个分离株亲缘关系密切, 元江分离株的10个管家基因串联分析结果显示与16SrⅠ-B亚组成员关系较近。4个地区的山黄麻丛枝病应是由同一种植原体所引起。本研究补充了植原体的基础数据, 为深入研究山黄麻植原体流行和防治奠定了良好基础。 |
| 英文摘要: |
| In recent years, Trema levigata witches’-broom disease has been continuously reported in different regions of Yunnan province, China. Infected plants exhibit typical witches’-broom and little leaf symptoms, causing severe damage. To confirm the pathogen of this disease, determine its taxonomic status, and clarify the phylogenetic relationships among isolates, PCR amplification of the 16S rRNA gene and ten housekeeping genes (rp, tuf, secA, secY, ipt, dnaK, fusA, gyrB, pyrG, and rpoB) was conducted for phylogenetic analysis. In addition, classification and identification of the isolates were performed using the online phytoplasma tool iPhyClassifier. The results showed that the pathogens causing T.levigata witches’-broom disease in Yuanjiang, Yimen, Xinping, and Eshan of Yunnan all belonged to ‘Candidatus Phytoplasma asteris’-related strains and were members of the phytoplasma 16SrⅠ-Ⅹ subgroup. Phylogenetic trees based on the fusA, gyrB, and rpoB genes showed that the four isolates were closely related. Concatenated analysis of the ten housekeeping genes of the Yuanjiang isolate indicated that it was more closely related to members of the 16SrⅠ-B subgroup. Therefore, the witches’-broom disease of T.levigata in the four regions was likely caused by the same phytoplasma. This study supplements the basic data on phytoplasmas and lays a solid foundation for further in-depth research on the epidemiology and control of T.levigata phytoplasma disease. |
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