| 董 汝 1#, 尹跃艳 2#, 钟 静 1, 赵丽玲 1, 林 涛 1, 李婷婷 1*, 丁 铭 1*.侵染云南省滇红花的黄瓜花叶病毒的基因组特征及致病性分析[J].植物保护,2026,52(4):66-75. |
| 侵染云南省滇红花的黄瓜花叶病毒的基因组特征及致病性分析 |
| Genomic characterization and pathogenicity assessment of cucumber mosaic virus infecting safflower in Yunnan province |
| 投稿时间：2025-08-11 修订日期：2025-11-07 |
| DOI：10.16688/j.zwbh.2025421 |
| 中文关键词: 红花 黄瓜花叶病毒 卫星RNA 药用植物 |
| 英文关键词:safflower cucumber mosaic virus satellite RNA medicinal plant |
| 基金项目:云南省种子种业联合实验室项目(202205AR070001-02);云南省重大科技专项计划(202302AE090005-6);云南省乡村振兴科技专项(200304BI090032-9);云南省农业科学院生物技术与种质资源研究所开放项目(2025002) |
| 作者 | 单位 | E-mail | | 董 汝 1#, 尹跃艳 2#, 钟 静 1, 赵丽玲 1, 林 涛 1, 李婷婷 1*, 丁 铭 1* | 1. 云南省农业科学院生物技术与种质资源研究所, 云南省农业生物技术重点实验室, 农业农村部西南农业基因资源与种质创制重点实验室, 昆明 650223
2. 云南省农业科学院高山经济植物研究所, 丽江 674100 | 李婷婷591807832@qq.com;丁铭mingd73@163.com |
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| 中文摘要: |
| 黄瓜花叶病毒(cucumber mosaic virus, CMV)属于雀麦花叶病毒科 Bromoviridae 黄瓜花叶病毒属Cucumovirus, 具有广泛的寄主范围, 可侵染 100个科、521个属中的1 071个物种。红花 Carthamus tinctorius 是一种重要的药用植物, CMV 在全球红花植物中广泛流行, 危害严重。本研究于云南省采集表现黄化、矮化和芽枯症状的滇红花植株, 通过小RNA测序和 RT-PCR 扩增, 明确其中3株感染了CMV, 命名为 CMV-crocus。并获得了 CMV RNA1、RNA2 和 RNA3 的全基因组序列, 序列分析结果显示 CMV-crocus 的 2b 蛋白与其他CMV 的蛋白序列差异较大。进一步发现来自其他分离物的卫星 RNA 能够降低滇红花上 CMV-crocus 分离物的病毒积累量。本研究首次从云南滇红花上获得 CMV 的全长序列, 并对其致病性进行研究, 为后续滇红花的病毒防治提供了基础。 |
| 英文摘要: |
| Cucumber mosaic virus (CMV) belongs to the genus Cucumovirus in the family Bromoviridae and has a wide host range, infecting 1 071 species in 521 genera across 100 families. Carthamus tinctorius (safflower) is an important medicinal plant, and CMV is widely prevalent in safflower worldwide, causing severe damage. In this study, safflower plants exhibiting symptoms of chlorosis, dwarfing, and bud blight were collected in Yunnan province for virus detection. Through small RNA sequencing and RT-PCR amplification, three CMV-infected plants were identified, and the complete genome sequences of CMV RNA1, RNA2, and RNA3 were obtained and designated as CMV-crocus. Sequence analysis showed that the 2b protein of CMV-crocus differed greatly from those of other CMV isolates. Further investigations revealed that satellite RNAs from other isolates could reduce the viral accumulation of CMV-crocus in safflower. This study is the first to obtain the full-length genome sequence of CMV from safflower in Yunnan and to investigate its pathogenicity, providing a basis for subsequent virus control and prevention in safflower. |
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