| 滕海媛1#, 顾浩天1#, 王冬生1, 张颂函2, 张天澍1, 袁永达1*.甜菜夜蛾短神经肽F基因调控取食、能量代谢及饥饿耐受性的功能分析[J].植物保护,2026,(1):39-46. |
| 甜菜夜蛾短神经肽F基因调控取食、能量代谢及饥饿耐受性的功能分析 |
| Functional analysis of short neuropeptide F gene regulating feeding, energy metabolism and starvation tolerance in Spodoptera exigua |
| 投稿时间：2025-02-07 修订日期：2025-08-07 |
| DOI：10.16688/j.zwbh.2025056 |
| 中文关键词: 甜菜夜蛾 短神经肽 F 取食 糖代谢 |
| 英文关键词:Spodoptera exigua short neuropeptide F feeding sugar metabolism |
| 基金项目:上海市现代农业产业技术体系建设[沪农科产字（2024）第1号] |
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| 中文摘要: |
| 为明确甜菜夜蛾Spodoptera exigua短神经肽F(sNPF)基因对其取食行为、能量代谢及饥饿耐受性的调控作用, 本研究克隆并分析了该基因功能。结果表明:甜菜夜蛾sNPF基因开放阅读框(ORF)为548 bp, 编码182个氨基酸; 其蛋白序列与鳞翅目夜蛾科其他物种sNPF蛋白氨基酸序列相似性达96.7%~100%, 具有高度保守性。组织表达分析显示, sNPF在4龄幼虫期表达量最高, 且在头部特异性高表达。通过RNA干扰技术(注射双链sNPF干扰RNA, dssNPF)研究发现:与对照(注射dsGFP)相比, 干扰后24、48、72 h甜菜夜蛾4龄幼虫取食量分别显著降低13.15%、17.59%和20.13%; 同期体重累计增长率分别显著下降10.14%、23.08%和34.59%。饥饿胁迫试验进一步揭示, 干扰sNPF基因导致4龄幼虫48 h和72 h死亡率显著升高至54%和100%; 同时, 幼虫体内糖原含量显著减少37.53%, 而海藻糖(升高66.42%)、海藻糖合成酶(升高24.26%)、胰岛素(升高53.59%)含量均显著上升。上述结果提示, sNPF基因通过调控取食行为、能量物质积累与代谢途径(如糖原消耗加速、海藻糖合成增强等), 直接影响甜菜夜蛾的生长发育及饥饿耐受能力, 在其生存适应过程中发挥关键作用。本研究为靶向sNPF基因开发新型甜菜夜蛾绿色防控技术提供了重要理论依据。 |
| 英文摘要: |
| To clarify regulatory effects of short neuropeptide F (sNPF) gene in Spodoptera exigua on feeding behavior, energy metabolism and starvation tolerance, we cloned and functionally analyzed the sNPF gene. The results showed that the open reading frame (ORF) of the sNPF gene in S.exigua was 548 bp, encoding 182 amino acids. The protein sequence exhibited 96.7%-100% amino acid sequence homology with sNPF proteins from other species of the Noctuidae family within Lepidoptera, demonstrating high conservation. Tissue expression analysis revealed that sNPF was most highly expressed in the fourth-instar larvae, particularly in the head. RNA interference (RNAi) technology (via injection of double-stranded sNPF interfering RNA, dssNPF) was employed, and the results showed that compared with the control group (injected with dsGFP), the feeding amount of fourth-instar larvae was significantly reduced by 13.15%, 17.59% and 20.13% at 24, 48 and 72 h after interference, respectively. Meanwhile, the cumulative body weight during the same periods decreased significantly by 10.14%, 23.08%, and 34.59%, respectively. Further starvation stress experiments revealed that interference with the sNPF gene led to a significant increase in the mortality of fourth-instar larvae, reaching 54% and 100% at 48 and 72 h, respectively. In addition, the glycogen content in the larvae decreased significantly by 37.53%, while the levels of trehalose (increased by 66.42%), trehalose synthase (increased by 24.26%), insulin (increased by 53.59%) all increased significantly. These results suggest that the sNPF gene directly influences the growth, development and starvation tolerance of S.exigua by regulating feeding behavior and energy substance accumulation and metabolism (e.g., accelerated glycogen consumption and enhanced trehalose synthesis). This gene plays a key role in the survival and adaptation of the insect. The findings provide an important theoretical basis for developing novel and green control technologies targeting the sNPF gene for S.exigua management. |
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