| 何旭诺1, 黄娇芬2, 胡淑青1, 林 莉1, 李献锋1, 魏 霜1, 徐 强1, 武目涛1, 刘海军1*.基于形态和DNA条形码的小火蚁检疫鉴定[J].植物保护,2025,51(4):298-307. |
| 基于形态和DNA条形码的小火蚁检疫鉴定 |
| Quarantine identification of the little fire ant Wasmannia auropunctata based on morphology and DNA barcoding |
| 投稿时间：2024-09-12 修订日期：2025-03-04 |
| DOI：10.16688/j.zwbh.2024482 |
| 中文关键词: 小火蚁 形态特征 DNA条形码 鉴定 入侵种 |
| 英文关键词:Wasmannia auropunctata morphological identification DNA barcoding identification invasive species |
| 基金项目:国家重点研发计划（2022YFC2601500）；广州市重点研发计划（2023B04J0154）；广州海关科研项目（2023GZCK02） |
| 作者 | 单位 | E-mail | | 何旭诺1, 黄娇芬2, 胡淑青1, 林 莉1, 李献锋1, 魏 霜1, 徐 强1, 武目涛1, 刘海军1* | 1. 广州海关技术中心, 广州 510623
2. 佛山海关综合技术中心, 佛山 528000 | liuhj@iqtcnet.cn |
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| 中文摘要: |
| 小火蚁Wasmannia auropunctata原产于新热带地区, 被世界自然保护联盟(International Union for Conservation of Nature, IUCN)列为全球最具威胁的入侵物种之一。2022年, 小火蚁在广东汕头地区首次被发现, 成为中国大陆一种新发现的重大外来入侵有害生物。为实现快速、准确鉴定小火蚁, 本研究采用形态学方法对小火蚁标本进行分析, 编译了沃氏蚁属Wasmannia 10种工蚁和10种蚁后的检索表, 并利用DNA条形码技术对形态学鉴定结果进行了验证。本研究为小火蚁的准确鉴定提供了重要的技术参考。 |
| 英文摘要: |
| The little fire ant Wasmannia auropunctata, originally native to the Neotropical region, has been listed by the International Union for Conservation of Nature (IUCN) as one of the world’s most threatening invasive alien species. In 2022, W. auropunctata was detected for the first time in Shantou, Guangdong province, marking its emergence as a newly identified major invasive pest species in China’s mainland. To enable rapid and accurate identification of this species, a morphological method was employed to examine W.auropunctata specimens and dichotomous keys were compiled for workers and queen ants of 10 species within the genus Wasmannia. Additionally, DNA barcoding was subsequently used to verify the morphological identification. This study provides a valuable technical reference for the precise quarantine identification of W.auropunctata. |
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