| 邢晓芳1, 胡继辉1, 周 晨1, 罗 宁1, 刘变变1, 郭 炜1, 刘永刚2, 李惠霞1*.甘肃省多序岩黄芪孢囊线虫病病原鉴定[J].植物保护,2025,51(4):174-181. |
| 甘肃省多序岩黄芪孢囊线虫病病原鉴定 |
| Identification of the cyst nematode pathogen on Hedysarum polybotrys in Gansu province |
| 投稿时间：2024-09-19 修订日期：2024-10-08 |
| DOI：10.16688/j.zwbh.2024491 |
| 中文关键词: 甘肃 多序岩黄芪 大豆孢囊线虫 rDNA-ITS 28S rDNA D2/D3 |
| 英文关键词:Gansu Hedysarum polybotrys Heterodera glycines rDNA-ITS 28S rDNA D2/D3 |
| 基金项目:国家自然科学基金（32260654）；甘肃省重点研发计划-国际合作领域（24YFWA015） |
| 作者 | 单位 | E-mail | | 邢晓芳1, 胡继辉1, 周 晨1, 罗 宁1, 刘变变1, 郭 炜1, 刘永刚2, 李惠霞1* | 1. 甘肃农业大学植物保护学院, 甘肃省农作物病虫害生物防治工程实验室, 兰州 730070
2. 甘肃省农业科学院植物保护研究所, 兰州 730070 | lihx@gsau.edu.cn |
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| 中文摘要: |
| 为明确甘肃省陇南市特色中药材多序岩黄芪Hedysarum polybotrys孢囊线虫病的病原种类, 通过形态学与分子生物学相结合的方法对其进行鉴定, 采用室内人工接种的方法观察其生活史并测定对多序岩黄芪的致病性。结果表明, 该线虫的孢囊阔柠檬形, 双半膜孔, 下桥发达, 群体形态测量值与大豆孢囊线虫Heterodera glycines基本一致; 该种群rDNA-ITS序列与NCBI数据库中的大豆孢囊线虫相似性为99.8%, 与序列号为HM560787的大豆孢囊线虫聚在一起, 置信度为99%。28S-rDNA D2/D3序列与NCBI数据库中的大豆孢囊线虫相似性为100.00%, 与序列号为GU595446的大豆孢囊线虫聚在一起, 置信度为100%。因此, 形态学特征结合rDNA-ITS和28S-rDNA D2/D3序列, 将多序岩黄芪孢囊线虫病病原鉴定为大豆孢囊线虫。接种该线虫后的多序岩黄芪植株发育不良, 瘦弱矮小; 25℃下, 接种24 d根部开始出现白雌虫, 并在30 d产生大量白雌虫, 该世代基本完成, 33 d后形成孢囊。综上所述, 甘肃省陇南市寄生多序岩黄芪的孢囊线虫种类为大豆孢囊线虫, 该线虫可侵染多序岩黄芪并完成生活史, 对陇南市优势特色中药材多序岩黄芪的生产构成威胁。 |
| 英文摘要: |
| To identify the cyst nematode species infecting the medicinal plant Hedysarum polybotrys in Longnan city, Gansu province, a combined morphological and molecular approach was employed. The nematode’s life cycle and pathogenicity on H.polybotrys were evaluated through artificial inoculation under laboratory conditions. The results showed that the cysts were broad lemon-shaped with ambifenestrate fenestrae and well-developed underbridges. The morphological measurements were basically consistent with those of Heterodera glycines. The rDNA-ITS sequence of the population shared 99.8% identity with H.glycines in the NCBI database and clustered with accession HM560787 (bootstrap: 99%). The 28S rDNA D2/D3 region showed 100% identity with H.glycines (GU595446, bootstrap: 100%). Thus, based on morphological characteristics and molecular evidence (ITS and D2/D3 sequences), the nematode was identified as H.glycines. Infected H.polybotrys plants exhibited stunted and weakened growth. At 25℃, white females first appeared on the roots 24 days post-inoculation, with numerous white females observed at 30 days, indicating completion of one generation. Cysts formed 33 days post-inoculation. These findings confirm that H.glycines is capable of infecting H.polybotrys and completing its life cycle, posing a potential threat to the production of this valuable medicinal crop in Longnan city. |
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