| 蔡 霓1#, 乔治君1, 2#, 刘 芳1, 方 伟1, 刘少芳3, 农向群4, 王开梅1*.几株分离自麻天牛的白僵菌的生长、产孢特性及产酶能力分析[J].植物保护,2025,51(4):162-173. |
| 几株分离自麻天牛的白僵菌的生长、产孢特性及产酶能力分析 |
| Characteristics of growth, sporulation and enzyme production capacity of Beauveria strains isolated from Thyestilla gebleri |
| 投稿时间：2024-10-03 修订日期：2024-11-29 |
| DOI：10.16688/j.zwbh.2024523 |
| 中文关键词: 白僵菌 产孢 几丁质酶 蛋白酶 筛选 |
| 英文关键词:Beauveria spp. sporulation chitinase protease strain selection |
| 基金项目:湖北洪山实验室开放课题（2021hskf016）；湖北省重点研发专项（2023BBB176） |
| 作者 | 单位 | E-mail | | 蔡 霓1#, 乔治君1, 2#, 刘 芳1, 方 伟1, 刘少芳3, 农向群4, 王开梅1* | 1. 湖北省生物农药工程研究中心, 武汉 430064
2. 武汉轻工大学生命科学与技术学院, 武汉 430030
3. 天然微生物药物研究江西省重点实验室, 南昌 330013
4. 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193 | kaimei.wang@nberc.com |
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| 中文摘要: |
| 昆虫病原真菌白僵菌Beauveria spp.是害虫生防产品研发的主要真菌之一, 其生长及产酶特性是高毒力菌株筛选的重要依据。本研究对15株分离自麻天牛僵虫的白僵菌进行了鉴定, 并对其生长、产孢以及胞外水解酶活性进行了测定分析。rDNA-ITS序列进化关系分析结果显示, 15株白僵菌中11株为球孢白僵菌B. bassiana, 4株为硫酸白僵菌B. sulfurescens。不同温度及培养基条件下菌株生长与产孢分析结果表明, 25℃, PPDA培养基适合大部分白僵菌的生长, 温度下降至20℃时各菌株产孢量更高; CN004、CN011、CN012及CN055菌株为产孢优势候选菌株, 其中CN055菌株产孢量可达到(1.99±0.57)×106 个/mm2。胞外水解酶活性测定结果表明, CN006、CN012、CN040及CN055的几丁质酶活性较强, 且几丁质酶活性呈现先上升后下降趋势, 培养至第5天酶活性最高。CN009、CN019、CN040及CN055菌株的蛋白酶活性较强, 蛋白酶活性呈现先急剧上升、再平缓上升的趋势, CN055菌株第9 天的酶活性为(472.5±0.39) U/mL, 显著高于其他菌株(P<0.05)。CN040和CN055菌株在产孢、产几丁质酶和蛋白酶能力上兼具优势, 可作为后续研发的重点菌株。 |
| 英文摘要: |
| The entomopathogenic fungus Beauveria spp. is one of the most important fungal genera for the development of biocontrol agents. Growth and enzyme production traits are key indicators for selecting high-virulence strains. In this study, 15 Beauveria strains were isolated from naturally infected Thyestilla gebleri and identified via rDNA-ITS sequence analysis. Among them, 11 strains were identified as B. bassiana, and four as B.sulfurescens. The effects of temperature and media on fungal growth and sporulation were evaluated. Most strains exhibited optimal growth at 25℃ on PPDA medium, while sporulation peaked at 20℃. Strains CN004, CN011, CN012 and CN055 exhibited superior sporulation, with CN055 producing up to (1.99±0.57)×106 conidia/mm2. Extracellular enzyme activity assays showed that CN006, CN012, CN040 and CN055 had strong chitinase activity, which peaked on the 5th day after inoculation before declining. CN009, CN019, CN040 and CN055 showed high protease activity, with a sharp initial increase followed by a slower rise. On the 9th day, CN055 displayed the highest protease activity (472.5±0.39 U/mL), significantly higher than other strains (P<0.05). In summary, CN040 and CN055 demonstrated superior traits in terms of sporulation and hydrolytic enzyme production, making them promising candidates for future development of fungal biopesticides. |
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