| 谢明惠, 钟永志, 林璐璐, 张光玲, 赵 伟, 陈浩梁*.点蜂缘蝽滞育和非滞育对嗅觉相关基因表达的影响[J].植物保护,2025,51(4):98-108. |
| 点蜂缘蝽滞育和非滞育对嗅觉相关基因表达的影响 |
| Effects of diapause and non-diapause on the expression of olfactory-related genes in Riptortus pedestris |
| 投稿时间：2024-08-12 修订日期：2024-09-03 |
| DOI：10.16688/j.zwbh.2024416 |
| 中文关键词: 点蜂缘蝽 触角转录组 生殖滞育 嗅觉相关基因 差异表达基因 |
| 英文关键词:Riptortus pedestris antennal transcriptome reproductive diapause olfactory-related genes differentially expressed genes |
| 基金项目:国家重点研发计划（2023YFD1401000）；安徽省农业科技成果转化项目（2024ZH005） |
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| 中文摘要: |
| 有研究表明滞育和非滞育点蜂缘蝽Riptortus pedestris对聚集信息素的感受存在显著差异, 本研究拟通过测定滞育和非滞育点蜂缘蝽触角转录组, 寻找表达差异的嗅觉相关基因, 分析与滞育关联的信息素识别机制。利用高通量测序平台BGISEQ-500对滞育和非滞育点蜂缘蝽雌、雄成虫触角进行转录组测序, 筛选差异表达基因并进行通路富集分析, 同时对差异表达的嗅觉基因进行RT-qPCR验证。点蜂缘蝽触角转录组共获得57 648条unigenes, 其中61.05%在七大公共数据库中成功注释。注释到NR数据库的基因最多(52.90%), 匹配到茶翅蝽Halyomorpha halys的序列最多, 为55.83%。通过转录组数据分析, 与非滞育雌虫相比, 滞育雌虫触角中共筛选到2 222个差异表达基因, 其中上调表达基因1 288个, 下调表达基因934个; 与非滞育雄虫相比, 滞育雄虫触角中共筛选到2 293个差异表达基因, 其中上调表达基因1 410个, 下调表达基因883个; 雌、雄虫触角间差异基因较少, 分别为315个(滞育)和584个(非滞育)。进一步对嗅觉相关基因进行分析发现:滞育和非滞育雌虫触角间10个化学感受蛋白(chemosensory proteins, CSPs)基因和11个气味结合蛋白(odorant-binding proteins, OBPs)基因差异表达; 滞育和非滞育雄虫触角有7个CSPs基因和11个OBPs基因差异表达; 滞育雌、雄成虫仅有1个OBP基因表达出现差异; 非滞育雌、雄虫间有4个CSPs基因和3个OBPs基因差异表达。从差异表达的嗅觉相关基因中选取10个基因(4个OBPs，4个CSPs 2个ORs)进行RT-qPCR验证, 结果与转录组测序相符。研究结果将为今后点蜂缘蝽识别聚集信息素的分子机制及研发更为高效的化学通讯调控技术提供参考。 |
| 英文摘要: |
| Previous studies have shown significant differences in the response to aggregation pheromones between diapause and non-diapause individuals of Riptortus pedestris. To explore the molecular mechanisms underlying pheromone recognition associated with diapause, the antennal transcriptomes of diapause and non-diapause male and female adults were sequenced using the BGISEQ-500 high-throughput sequencing platform, screened for differentially expressed genes (DEGs), performed pathway enrichment analysis, and validated differentially expressed olfactory-related genes by RT-qPCR. A total of 57 648 unigenes were obtained from the antennal transcriptome, with 61.05% successfully annotated in seven public databases. The highest annotation rate was to the NR database (52.90%), with most matches corresponding to Halyomorpha halys (55.83%). Transcriptome analysis identified 2 222 DEGs between diapause and non-diapause females (1 288 up-regulated, 934 down-regulated) and 2 293 DEGs between diapause and non-diapause males (1 410 up-regulated, 883 down-regulated). Comparatively fewer DEGs were found between sexes within the same diapause status: 315 DEGs for diapause and 584 DEGs for non-diapause groups. Analysis of olfactory-related genes revealed that ten chemosensory protein (CSP) genes and eleven odorant-binding protein (OBP) genes were differentially expressed between diapause and non-diapause females, while seven CSP genes and eleven OBP genes were differentially expressed between diapause and non-diapause males. Only one OBP gene was differentially expressed between diapause females and males, whereas four CSP genes and three OBP genes differed between non-diapause females and males. Ten olfactory-related genes (four OBP genes, four CSP genes) and two OR genes were selected for RT-qPCR validation, and the results were consistent with the transcriptome data. These results provide a reference for understanding the molecular mechanisms of pheromone recognition in R.pedestris and support the development of more efficient chemical communication regulation technologies. |
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