| 佘小漫1#*, 李志芳2#, 马紫君1, 蓝国兵1, 丁善文1, 何自福1*.粉葛茎腐病病原鉴定及其根际细菌群落分析[J].植物保护,2025,51(4):88-97. |
| 粉葛茎腐病病原鉴定及其根际细菌群落分析 |
| Identification of the stem rot pathogen and rhizosphere bacterial community analysis in Pueraria montana var. thomsonii |
| 投稿时间：2024-08-23 修订日期：2024-09-23 |
| DOI：10.16688/j.zwbh.2024438 |
| 中文关键词: 粉葛 茎腐病 病原鉴定 高通量测序 根际 细菌群落 |
| 英文关键词:Pueraria montana var. thomsonii stem rot disease pathogen identification high-throughput sequencing rhizosphere bacterial community |
| 基金项目:科技创新战略专项资金(高水平农科院建设)(R2023PY-JG011)；2023年高水平广东省农业科技示范市建设资金项目(佛山粉葛根腐病防控关键技术示范与推广) |
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| 中文摘要: |
| 近年来粉葛茎腐病发生日趋增多, 严重影响粉葛的产量及质量。本研究从粉葛茎腐病病样中分离病原菌，根据其生理生化特征及分子生物学鉴定结果将其鉴定为须芒草罗布斯氏菌Robbsia andropogonis，革兰氏阴性。基于细菌16S rRNA, 应用高通量测序技术, 从健康粉葛和发病粉葛的根际土壤样品共获得613 254 条16S rRNA基因有效序列, 1 374个OTU, 分属于27门、53纲、100目、144科、213属、73种。发病粉葛和健康粉葛根际土壤之间细菌群落Alpha多样性差异显著, 但Beta多样性无显著差异。与健康粉葛根际土壤(FgJK)相比, 发病粉葛根际土壤(FgbJ)中蓝细菌门Cyanobacteriota和放线菌门Actinomycetota细菌的相对丰度显著降低, 假单胞菌门Pseudomonadota细菌的相对丰度显著提高; 在属水平上, 副伯克霍尔德氏菌属Paraburkholderia、泛菌属Pantoea、假单胞菌属Pseudomonas和根瘤菌属Rhizobium细菌的相对丰度显著提高; 鞘氨醇单胞菌属Sphingomonas细菌的相对丰度显著降低。 |
| 英文摘要: |
| Stem rot has become increasingly prevalent in Pueraria montana var. thomsonii, severely affecting its yield and quality. This study aimed to identify the causal pathogen and evaluate its effects on the rhizosphere bacterial community to support effective disease management strategies. The pathogen isolated from diseased stems was identified as the Gram-negative bacterium Robbsia andropogonis based on physiological, biochemical and molecular analyses. Using high-throughput 16S rRNA gene sequencing, a total of 613 254 high-quality sequences and 1 374 OTUs were obtained from the rhizosphere soils of healthy and diseased plants, encompassing 27 phyla, 53 classes, 100 orders, 144 families, 213 genera and 73 species. Alpha diversity showed significant differences between the two groups, while Beta diversity did not. Compared to healthy rhizosphere soils (FgJK), the diseased soils (FgbJ) exhibited significantly lower relative abundances of Cyanobacteriota and Actinomycetota, and a significantly higher abundance of Pseudomonadota. At the genus level, the relative abundances of Paraburkholderia, Pantoea, Pseudomonas, and Rhizobium were significantly higher in diseased soils, whereas Sphingomonas was significantly decreased. |
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