| 智亚楠, 尤伟晨, 李 芸, 李宇洁, 王春生, 陈利军*.L-香芹酮对灰葡萄孢的熏蒸抑菌活性及抑菌机制初探[J].植物保护,2025,51(4):63-71. |
| L-香芹酮对灰葡萄孢的熏蒸抑菌活性及抑菌机制初探 |
| Preliminary investigation of the fumigation antifungal activity and mechanisms of L-carvone against Botrytis cinerea |
| 投稿时间：2024-07-05 修订日期：2024-08-19 |
| DOI：10.16688/j.zwbh.2024363 |
| 中文关键词: L-香芹酮 熏蒸抑菌活性 灰葡萄孢 抑菌机制 |
| 英文关键词:L-carvone fumigation antifungal activity Botrytis cinerea mechanism of action |
| 基金项目:河南省自然科学基金（232300420030）；河南省高等学校重点科研项目(25B210017); 信阳农林学院作物绿色防控与品质调控科技创新团队（XNKJTD 007） |
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| 中文摘要: |
| 为明确L-香芹酮的熏蒸抑菌活性和其作用机制, 本研究利用菌丝生长速率法测定了L-香芹酮对灰葡萄孢、核盘菌、齐整小核菌、禾谷镰孢、胶孢炭疽菌5种植物病原菌的熏蒸抑制作用, 采用孢子萌发法进一步探究了L-香芹酮对灰葡萄孢的作用, 通过扫描电镜和透射电镜观察熏蒸处理后灰葡萄孢菌丝微观形态和亚细胞结构的变化, 利用PI染色观察细胞膜的损伤情况, 并测定L-香芹酮对灰葡萄孢麦角甾醇合成、细胞膜胞外电导率、蛋白质和核酸含量的影响, 开展了L-香芹酮对草莓灰霉病的离体防效试验。结果显示, L-香芹酮对5种植物病原菌菌丝生长有明显的抑制作用, 熏蒸72 h EC50在5.29~18.69 μL/L之间。对灰葡萄孢孢子萌发的EC50为11.78 μL/L; 经L-香芹酮熏蒸处理后灰葡萄孢菌丝干瘪, 扭曲, 细胞器结构紊乱; PI染色结果表明, L-香芹酮熏蒸处理严重影响灰葡萄孢细胞膜的完整性, EC50和EC90剂量处理组麦角甾醇含量分别降低27.3%和43.2%, 胞内物质外泄, 菌丝液的相对电导率上升, 蛋白质和核酸类物质外渗程度高于对照。EC90剂量熏蒸处理对草莓灰霉病治疗和保护防控效果分别为85.43%和90.31%。综上, L-香芹酮可通过破坏灰葡萄孢细胞膜的完整性和通透性来发挥抑菌功效, 对草莓灰霉病有较好的防治效果, 该结果为新型植物源熏蒸杀菌剂的开发提供了新思路。 |
| 英文摘要: |
| To elucidate the fumigation antifungal activity and underlying mechanism of L-carvone, its inhibitory effects on five plant pathogenic fungi (Botrytis cinerea, Sclerotinia sclerotiorum, Sclerotium rolfsii, Fusarium graminearum and Colletotrichum gloeosporioides) were investigated using the mycelial growth rate method. The impact on B. cinerea spore germination was assessed via spore germination assays. Morphological and ultrastructural changes in B. cinerea hyphae following L-carvone fumigation were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), while cell membrane integrity was evaluated using propidium iodide (PI) staining. Moreover, the effects of L-carvone on ergosterol synthesis, extracellular conductivity, and leakage of proteins and nucleic acids were also examined. In vitro assays were also conducted to assess its efficacy in controlling strawberry gray mold. The results showed that L-carvone had significant inhibitory effects on all five plant pathogens, with EC50 values ranging from 5.29 to 18.69 μL/L 72 h after fumigation. The EC50 for B.cinerea spore germination was 11.78 μL/L. B.cinerea hyphae appeared shriveled and distorted, with disorganized organelles after fumigation. PI staining confirmed significant damage to the cell membrane. Ergosterol content was reduced by 27.3% and 43.2% after treated with EC50 and EC90of L-carvone, respectively. Cytoplasmic leakage resulted in increased relative conductivity, as well as elevated protein and nucleic acid content in the mycelial exudate. EC90 treatment of L-carvone achieved 85.43% therapeutic and 90.31% protective control efficacy against strawberry gray mold. In conclusion, L-carvone inhibits B.cinerea primarily by disrupting cell membrane integrity and permeability, offering a promising strategy for developing plant-derived fumigant fungicides. |
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