| 刘一贤, 孔祥东, 岩香甩*.云南西双版纳珠芽黄魔芋棒孢霉叶斑病病原鉴定[J].植物保护,2025,51(3):313-317. |
| 云南西双版纳珠芽黄魔芋棒孢霉叶斑病病原鉴定 |
| Identification of the pathogen causing leaf spot disease of Amorphophallus muelleri in Xishuangbanna, Yunnan |
| 投稿时间：2024-06-20 修订日期：2024-07-19 |
| DOI：10.16688/j.zwbh.2024335 |
| 中文关键词: 珠芽黄魔芋 叶斑病 多主棒孢 病原鉴定 |
| 英文关键词:Amorphophallus muelleri leaf spot disease Corynespora cassiicola pathogen identification |
| 基金项目:云南省热带作物科学研究所热带作物科技创新专项资金(RF2024-2-1);西双版纳橡胶林生态系统云南省野外科学观测研究站(202205AM070004);云南省现代农业橡胶产业技术体系(2024KJTX-12-3);云南省基础研究计划农业联合专项面上项目(202401BD070001-124) |
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| 中文摘要: |
| 为明确云南西双版纳橡胶林下珠芽黄魔芋Amorphophallus muelleri上新发生的一种叶斑病的病原菌, 本研究采用组织分离法进行病原菌分离, 有伤接种和无伤接种两种方法进行致病性测定, 通过形态学观察结合rDNA-ITS和tef1序列联合构建系统发育树明确病原种类。结果表明, 该病原菌为多主棒孢Corynespora cassiicola, 是珠芽黄魔芋叶斑病病原菌新记录种, 本研究结果为后续珠芽黄魔芋叶斑病的研究奠定了基础。 |
| 英文摘要: |
| To identify the pathogen responsible for a newly occurring leaf spot disease on Amorphophallus muelleri in Xishuangbanna, Yunnan province, a tissue isolation method was employed to culture and isolate the pathogen. Pathogenicity of the isolate was assessed using non-wounded and wounded inoculation methods. Classification status of the isolate was identified through morphological characteristics, rDNA-ITS and tef1 gene sequence analysis. The results showed that identified the pathogen causing leaf spot disease on A.muelleri in Xishuangbanna was Corynespora cassiicola. This pathogen was a new record of A.muelleri leaf spot, which offered valuable insights for future studies on the disease. |
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