| 侯 璐*, 方世玉, 康胜华, 龙 有.青稞条纹病原菌的接种方法优化和利用[J].植物保护,2025,51(3):276-281. |
| 青稞条纹病原菌的接种方法优化和利用 |
| Optimization and utilization of an inoculation method for the hulless barley stripe pathogen |
| 投稿时间：2024-04-16 修订日期：2024-12-22 |
| DOI：10.16688/j.zwbh.2024217 |
| 中文关键词: 青稞 条纹病 液体培养基 抗病性评价 |
| 英文关键词:hulless barley leaf stripe liquid medium disease resistance evaluation |
| 基金项目:国家自然科学基金(32160647) |
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| 摘要点击次数: 24 |
| 全文下载次数: 12 |
| 中文摘要: |
| 条纹病是青藏高原青稞生产上的重要种传病害之一, 带菌种子是青稞条纹病的唯一初侵染菌源, 常采用三明治法幼芽接种病原菌, 但其试验周期长和对青稞种子的侵染效率较低。为提高人工接种条纹病病原菌的侵染效率, 本研究建立了液体培养条纹病病原菌菌丝体接种青稞种子的液体侵染接种方法, 确定液体侵染法的最适接种条件。研究发现，从整个试验周期看，液体侵染法耗时为三明治法的42.9%~57.9%，从青稞种子与病原菌的共培养时间来看，液体侵染法耗时为三明治法的7.9%。缩短人工接种处理时间18.42 d, 说明液体侵染法可作为室内接种鉴定青稞品种对条纹病抗性的有效方法。本研究采用液体侵染法对30份青稞种质资源进行抗病性鉴定, 获得免疫性遗传资源材料3份、高抗类型材料5份、中抗类型材料8份、中度感病类型材料3份、高度感病类型材料11份。本研究采用液体侵染接种方法提高病原菌侵染率, 缩短侵染处理时间, 对改进青稞条纹病菌接种具有重要意义。 |
| 英文摘要: |
| Stripe disease is one of the most important seed-borne diseases affecting hulless barley production on the Qinghai-Xizang Plateau. Infected seeds serve as the only primary inoculum source of the pathogen, and the commonly used sandwich method for inoculating young shoots with the pathogen has low infection efficiency and requires a long testing period. To improve the infection efficiency of artificial inoculation, this study established a liquid inoculation method, in which hulless barley seeds were inoculated with mycelia of the stripe disease pathogen cultured in liquid medium, and the optimal inoculation conditions were determined. The results showed that the whole test cycle of liquid infection method was 42.9%-57.9% of sandwich method and co-cultivation time was 7.9% of sandwich method, which means the artificial inoculation treatment time shortened by 18.42 d, indicating the liquid infection method can be used as an effective method for indoor inoculation to identify the resistance of hulless barley varieties to stripe disease. Using the liquid inoculation method, this study evaluated the disease resistance of 30 hulless barley germplasm resources, identifying 3 immune genetic resources, 5 highly resistant types, 8 moderately resistant types, 3 moderately susceptible types, and 11 highly susceptible types. By improving pathogen infection rates and shortening inoculation treatment times, the liquid inoculation method used in this study provides a significant advancement in the inoculation technique for stripe disease pathogens in hulless barley. |
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