| 王振花2#, 贾云梦1, 2#, 张美惠2, 徐 飞2, 3, 李松杰1, 2, 商昭月1, 2, 范洁茹2, 袁军海1*, 刘 伟2*, 周益林2.高温胁迫下小麦白粉菌钙网蛋白基因表达量[J].植物保护,2025,51(3):153-157. |
| 高温胁迫下小麦白粉菌钙网蛋白基因表达量 |
| Expression levels of the calreticulin gene in Blumeria graminis f.sp. tritici under high temperature stress |
| 投稿时间：2024-05-17 修订日期：2024-06-21 |
| DOI：10.16688/j.zwbh.2024279 |
| 中文关键词: 小麦白粉菌 钙网蛋白 热激处理 表达量 |
| 英文关键词:Blumeria graminis f.sp. tritici calreticulin heat shock treatment gene expression |
| 基金项目:国家重点研发计划(2024YFD1400400)；国家自然科学基金(31972226) |
| 作者 | 单位 | E-mail | | 王振花2#, 贾云梦1, 2#, 张美惠2, 徐 飞2, 3, 李松杰1, 2, 商昭月1, 2, 范洁茹2, 袁军海1*, 刘 伟2*, 周益林2 | 1. 河北北方学院农林科技学院, 张家口 075000
2. 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193
3. 河南省农业科学院植物保护研究所, 农业农村部华北南部作物有害生物综合治理重点实验室, 郑州 450002 | 袁军海yuanjunhai@163.com;刘伟wliusdau@163.com |
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| 中文摘要: |
| 由专性寄生菌Blumeria graminis f.sp. tritici侵染引起的小麦白粉病现已成为我国小麦生产上的主要病害之一。为探究钙网蛋白(calreticulin, CRT)基因在小麦白粉菌抵御高温胁迫中的作用, 本研究筛选出温度敏感性菌株13-1-5-5-1-②、13-10-5-3-①、13-10-5-1-①和耐高温菌株13-14-1-②、13-14-8-2-②、13-10-5-3-②, 并对其在24℃热激处理下钙网蛋白CRT基因的表达量进行了研究。结果表明, 小麦白粉菌钙网蛋白gDNA序列测序全长为1 887 bp, cDNA序列1 650 bp, 具有完整的开放阅读框, 编码549个氨基酸, 含有钙网蛋白家族保守结构域，1～21位氨基酸为信号肽。在24℃热激处理后, 多数情况下温度敏感菌株和耐高温菌株CRT基因的表达量要高于未经高温处理的表达量, 最高可达40.56倍;同一菌株处理不同时间, CRT基因的表达量有一定差异, 且多数处理时间下耐高温菌株CRT基因相对表达量要高于敏感菌株, 这些研究结果对于深入了解白粉菌耐高温菌株的产生和对其进行有效监测具有重要意义。 |
| 英文摘要: |
| Wheat powdery mildew caused by the obligate parasite Blumeria graminis f.sp. tritici (Bgt) has become one of the major wheat diseases in China. To clarify the calreticulin (CRT) gene in Bgt’s resistance to high-temperature stress, six isolates were screened, including the temperature-sensitive isolates (13-1-5-5-1-②, 13-10-5-3-①, 13-10-5-1-①) and temperature-tolerant isolates (13-14-1-②, 13-14-8-2-②, 13-10-5-3-②). The expression levels of the CRT gene under heat shock conditions (24℃) were analyzed. The results showed that the full-length gDNA sequence of the Bgt CRT gene was 1 887 bp, while its cDNA sequence was 1 650 bp, containing a complete open reading frame encoding 549 amino acids. The first 21 amino acids were predicted as a signal peptide, and a conserved calreticulin domain was identified. Under high-temperature stress, the CRT gene expression increased in most cases, with expression levels in temperature-sensitive and temperature-tolerant isolates exceeding those of untreated controls. The highest expression level reached 40.56-fold. The expression levels varied over time within the same isolate, but temperature-tolerant isolates consistently exhibited higher CRT gene expression compared to temperature-sensitive isolates in most treatments. These findings are of great significance for deepening the understanding of the molecular mechanisms of Bgt high-temperature-tolerant isolates and facilitating their effective monitoring. |
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