| 田 沺1, 2, 林祥胜1, 2, 丁夏威2, 3, 汤法江2, 3, 邹宏达2, 王章英2, 梁雪莲1*, 黄立飞2*.茎腐病胁迫下甘薯CC-NBS-LRR基因家族鉴定及转录组分析[J].植物保护,2025,51(3):122-134. |
| 茎腐病胁迫下甘薯CC-NBS-LRR基因家族鉴定及转录组分析 |
| Identification of the CC-NBS-LRR gene family in sweet potato and transcriptome analysis under Dickeya dadantii infection |
| 投稿时间：2024-04-04 修订日期：2024-11-28 |
| DOI：10.16688/j.zwbh.2024185 |
| 中文关键词: 甘薯 CC-NBS-LRR基因家族 生物信息学 茎腐病 |
| 英文关键词:sweet potato CC-NBS-LRR gene family bio-informatics bacterial stem and root rot disease |
| 基金项目:广东省科技计划(2023B0202010019);国家甘薯产业技术体系(CARS-10) |
| 作者 | 单位 | E-mail | | 田 沺1, 2, 林祥胜1, 2, 丁夏威2, 3, 汤法江2, 3, 邹宏达2, 王章英2, 梁雪莲1*, 黄立飞2* | 1. 仲恺农业工程学院, 广州 510640
2. 广东省农业科学院作物研究所, 广东省农作物遗传改良重点实验室, 广州 510640
3. 华南农业大学农学院, 广州 510640 | 梁雪莲 liangxuelian2005@sina.com;黄立飞 hlf157@163.com |
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| 中文摘要: |
| 由达旦提狄克氏菌Dickeya dadantii引起的细菌性病害是甘薯的主要病害之一, 是我国南方甘薯主产区发病面积最大, 危害最为严重的病害。本研究对参与响应茎腐病胁迫的甘薯CC-NBS-LRR基因家族进行鉴定, 并对其蛋白质理化性质、基因结构、保守基序、进化关系、染色体定位、顺式作用元件、表达部位等进行分析, 进一步通过转录组测序和荧光定量PCR分析CC-NBS-LRR基因在抗病品种‘广薯87’和感病品种‘心香’受茎腐病菌侵染后的表达差异。结果表明, 甘薯中有17个CC-NBS-LRR基因参与了茎腐病胁迫响应, 其编码蛋白长度为787~1 241 aa, 等电点为6.46~9.14, 主要分布在3号染色体上。它们与甘薯野生种Ipomoea trifida和I.triloba的CC-NBS-LRR基因聚类为一簇, 与拟南芥Arabidopsis thaliana的4个CC-NBS-LRR基因亲缘关系较近, 多数基因的启动子为响应光反应的顺式作用元件, 其中IbCNL11和IbCNL13基因在甘薯根、茎和叶中表达量较高, IbCNL1、IbCNL2和IbCNL11与茎腐病抗性密切相关。综上, 本研究明确了参与响应茎腐病胁迫的CC-NBS-LRR基因家族的特征、进化和表达模式, 筛选出3个抗病候选基因, 为解析甘薯抗茎腐病机制奠定了基础。 |
| 英文摘要: |
| The bacterial disease caused by Dickeya dadantii is one of the main diseases affecting sweet potato production, causing widespread damage in southern China. This study identified the CC-NBS-LRR gene family involved in the bacterial stem and root rot stress response, and analyzed their physicochemical properties, gene structures, conserved motifs, evolution, chromosome mapping, cis-acting elements and expression patterns. Additionally, transcriptome sequencing and quantitative PCR analysis were conducted to investigate expression differences in resistant variety (‘Guangshu 87’) and susceptible variety (‘Xinxiang’) following D.dadantii infection. The results showed that 17 CC-NBS-LRR genes were involved in the bacterial stem and root rot response; the encoded amino acid lengths ranging from 787 to 1 241 residues, with isoelectric points between 6.46 and 9.14, which were mainly located on chromosome 3. Phylogenetic analysis showed that the CC-NBS-LRR genes clustered with Ipomoea trifida and I.triloba, and were closely related to four CC-NBS-LRR genes from Arabidopsis thaliana. Most gene promoters contained cis-acting elements associated with light response, among which IbCNL11 and IbCNL13 genes were highly expressed in sweet potato roots, stems and leaves. IbCNL1, IbCNL2 and IbCNL11 were identified as key genes associated with bacterial stem rot resistance. In conclusion, this study clarifies the characteristics, evolution and expression patterns of the CC-NBS-LRR gene family in response to bacterial stem and root rot stress and identifies three candidate resistance genes. These findings provide a foundation for further understanding the mechanisms of bacterial stem rot resistance in sweet potato. |
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