| 陈志伟1, 程云霞2, 张 蕾2, 徐林波3, 江幸福2*, 孔海龙1*.章鱼胺对草地螟幼虫密度依赖抗病性的调控作用[J].植物保护,2025,51(3):56-61. |
| 章鱼胺对草地螟幼虫密度依赖抗病性的调控作用 |
| Density-dependent disease resistance in Loxostege sticticalis mediated by octopamine |
| 投稿时间：2024-11-21 修订日期：2025-01-20 |
| DOI：10.16688/j.zwbh.2024610 |
| 中文关键词: 草地螟 幼虫密度 抗病能力 章鱼胺 免疫功能 |
| 英文关键词:Loxostege sticticalis larval density disease resistance octopamine immune function |
| 基金项目:国家重点研发计划(2022YFD1400600);国家自然科学基金(32172397) |
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| 中文摘要: |
| 为阐明生物胺对草地螟Loxostege sticticalis幼虫密度依赖抗病性的调控作用, 在室内温度为(22±1)℃, 相对湿度为70%, 光周期为L∥D=16 h∥8 h条件下, 探究了不同密度:低密度(1头/瓶)、高密度(10头/瓶)、超高密度(30头/瓶)饲养的草地螟幼虫抗病能力和3种生物胺含量，以及外源生物胺对幼虫抗病免疫的影响。结果表明, 不同密度幼虫的抗病能力差异显著, 高密度(10头/瓶)幼虫对球孢白僵菌Beauveria bassiana的抵抗能力显著高于低密度(1头/瓶)和超高密度(30头/瓶)幼虫。高密度的幼虫血淋巴中章鱼胺含量明显高于其他2个密度。不同密度幼虫血淋巴中多巴胺和5-羟色胺的含量无显著变化。注射不同浓度(2、20、200 μg/mL)外源章鱼胺后, 幼虫被球孢白僵菌侵染后的存活率显著升高, 对球孢白僵菌的抵抗能力明显增强。注射20 μg/mL章鱼胺后, 幼虫的酚氧化酶活性和血细胞包囊作用明显增强, 血细胞总数显著增多, 血细胞包囊率显著增加。以上结果表明, 章鱼胺可调控草地螟幼虫的免疫功能以增强其抗病能力。因此, 章鱼胺在草地螟幼虫密度依赖抗病免疫中具有重要作用。 |
| 英文摘要: |
| To clarify the regulatory role of biogenic amines in density-dependent disease resistance in Loxostege sticticalis larvae, this study investigated disease resistance and biogenic amine levels of larvae reared at different larval densities: high density (10 larvae/jar), low density (1 larva/jar), and ultra-high density (30 larvae/jar) under controlled laboratory conditions ［temperature: (22 ± 1)℃, relative humidity: 70%, photoperiod: L∥D=16 h∥8 h］, and the effects of exogenous biogenic amines on the larval disease resistance and immunity. The results showed that disease resistance varied significantly across densities. High-density larvae (10 larvae/jar) exhibited significantly greater resistance to Beauveria bassiana than low-density larvae (1 larva/jar), while ultra-high-density larvae (30 larvae/jar) had significantly lower resistance. High-density larvae had significantly higher octopamine content than those at low-density and ultra-high-density. Dopamine and serotonin levels showed no significant differences among density groups. Exogenous octopamine injection enhanced disease resistance. Injection of 2, 20, 200 μg/mL octopamine increased survival rates following B. bassiana infection. Injection of 20 μg/mL octopamine resulted in increased phenoloxidase activity and hemocyte encapsulation, and a significant increase in total number of hemocytes and encapsulation rate. These results indicate that octopamine regulates the immune function of L. sticticalis larvae, enhancing disease resistance in high-density populations. Therefore, octopamine plays an important role in density-dependent disease resistance in L. sticticalis. |
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