| 潘秋波1, 杨 强1, 徐红星2, 郑许松2, 吕仲贤2, 鲁艳辉2*.浙江平湖地区二化螟田间种群对6种杀虫剂的抗性监测[J].植物保护,2025,51(2):297-303. |
| 浙江平湖地区二化螟田间种群对6种杀虫剂的抗性监测 |
| Resistance monitoring of Chilo suppressalis field populations to six insecticides in Pinghu, Zhejiang |
| 投稿时间：2024-05-03 修订日期：2024-06-23 |
| DOI：10.16688/j.zwbh.2024255 |
| 中文关键词: 二化螟 杀虫剂 抗性监测 生物测定 解毒酶 |
| 英文关键词:Chilo suppressalis insecticide resistance monitoring bioassay detoxification enzyme |
| 基金项目:农产品质量安全全国重点实验室开放课题(2021DG700024-KF202320); 平湖市农业农村局科技合作项目(PH20220003) |
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| 中文摘要: |
| 为明确平湖地区二化螟Chilo suppressalis田间种群的抗性水平及抗性机制, 连续2年测定了6个二化螟种群对6种杀虫剂的抗性, 并测定了上述种群的主要解毒酶羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GSTs)及多功能氧化酶(P450)活性。结果表明, 平湖地区二化螟种群对氯虫苯甲酰胺、四唑虫酰胺、阿维菌素、多杀霉素、甲氧虫酰肼产生了中高等水平抗性。相同年份比较, 3个不同田间种群对同一药剂的抗性水平差别不大; 不同年份比较, 抗药性变化情况随药剂不同而变化。二化螟种群对不同药剂抗药性与解毒酶活性的相关性分析结果显示, 氯虫苯甲酰胺、四唑虫酰胺、阿维菌素、多杀霉素4种药剂的抗性与P450酶活性具有显著相关性。由此可见, 平湖地区6个二化螟种群对所监测的6种常用药剂均产生了不同程度的抗药性, 且大部分药剂抗性的产生机制与P450酶活性相关。 |
| 英文摘要: |
| To clarify the resistance levels and mechanisms of Chilo suppressalis field populations in Pinghu, Zhejiang, this study investigated the resistance levels of six populations of C. suppressalis to six commonly used insecticides over two years. Additionally, the activities of major detoxification enzymes, including carboxylesterase (CarE), glutathione S-transferase (GSTs), and multifunctional oxidase (P450), were assessed. The results showed that moderate to high resistance levels were observed for chlorantraniliprole, tetraniliprole, abamectin, spinosad, and methoxyfenozide. Resistance levels to the same insecticide showed minimal variation among the three populations in the same year. The changes in resistance levels varied across different years depending on the type of pesticide used. Correlation analysis showed that resistance to chlorantraniliprole, tetraniliprole, abamectin, and spinosad was significantly correlated with P450 enzyme activity. In conclusion, the six C. suppressalis populations in Pinghu exhibited varying resistance levels to the monitored insecticides. The resistance mechanisms of most insecticides were primarily linked to P450 enzyme activity. |
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