| 胡连清1, 2, 刘雯雯1, 2, 冯瑞章1, 2*, 周万海1, 2, 查黎萍1, 唐馨宇1, 席万鹏3, 陈朝清4.4种微生物菌剂对为害油樟的黑绒鳃金龟死亡率、取食及代谢酶活性的影响[J].植物保护,2025,51(2):184-192. |
| 4种微生物菌剂对为害油樟的黑绒鳃金龟死亡率、取食及代谢酶活性的影响 |
| Effects of four microbial agents on the mortality rate, feeding behavior, and metabolic enzyme activities of Maladera orientalis infesting Camphora longepaniculata |
| 投稿时间：2024-03-13 修订日期：2024-04-28 |
| DOI：10.16688/j.zwbh.2024144 |
| 中文关键词: 黑绒鳃金龟 微生物菌剂 死亡率 保护酶 解毒酶 消化酶 |
| 英文关键词:Maladera orientalis microbial agent mortality protective enzyme detoxifying enzyme digestive enzyme |
| 基金项目:四川省自然科学基金(2022NSFSC0176); 宜宾市科技计划(2022NY018); 宜宾学院2021年校级培育项目(2021PY68); 四川省油樟工程技术研究中心开放基金(2021YZY06); 四川省油樟工程技术研究中心开放基金(2021YZY02, 2021XLZ01); 宜宾市科技局高端团队项目(2021YGC03) |
| 作者 | 单位 | E-mail | | 胡连清1, 2, 刘雯雯1, 2, 冯瑞章1, 2*, 周万海1, 2, 查黎萍1, 唐馨宇1, 席万鹏3, 陈朝清4 | 1. 宜宾学院农林与食品工程学部, 宜宾 644000
2. 四川省油樟工程技术研究中心, 宜宾 644000
3. 西南大学, 重庆 404100
4. 四川宸煜林业开发有限责任公司, 宜宾 644000 | ruizhangfeng@126.com |
|
| 摘要点击次数: 90 |
| 全文下载次数: 88 |
| 中文摘要: |
| 为探究球孢白僵菌Beauveria bassiana SW21、布氏白僵菌B.brongniartii SW22、苏云金芽胞杆菌Bacillus thuringiensis SW12及金龟子绿僵菌Metarhizium anisopliae SW23对为害油樟的黑绒鳃金龟Maladera orientalis的防控效果, 于菌剂处理后不同时间测定黑绒鳃金龟死亡率，取食量以及其肠道中过氧化物酶(peroxidase, POD)、超氧化物歧化酶(superoxide dismutase, SOD)及过氧化氢酶(catalase, CAT)3种保护酶、乙酰胆碱酯酶(acetylcholinesterase, AChE)、羧酸酯酶(carboxylesterase, CES)、多功能氧化酶(mixed-function oxidase, MFO)、细胞色素P450(cytochrome P450, CYP450)与谷胱甘肽硫转移酶(glutathione S-transferase, GST)5种解毒酶和淀粉酶(amylase, AMS)、胰蛋白酶(trypsin)与脂肪酶(lipase)3种消化酶活性。结果表明:4种微生物菌剂对黑绒鳃金龟死亡率和取食量的影响强弱依次为布氏白僵菌＞金龟子绿僵菌＞球孢白僵菌＞苏云金芽胞杆菌。4种微生物菌剂可不同程度地抑制黑绒鳃金龟肠道中SOD与CAT 2种保护酶活性, 促进虫体内POD、AChE、CES、CYP450、GST、MFO、AMS、脂肪酶和胰蛋白酶活性升高。研究结果表明, 4种微生物菌剂可通过持续抑制SOD与CAT的活性, 降低黑绒鳃金龟清除体内活性氧自由基的能力, 造成黑绒鳃金龟氧化损伤, 最终增加虫体死亡率; 而黑绒鳃金龟可通过上调POD、 AChE、CES、CYP450、GST、MFO、AMS、脂肪酶和胰蛋白酶的活性来维持其正常的生理功能, 4种微生物菌剂在黑绒鳃金龟绿色防控方面均具有一定的潜力。 |
| 英文摘要: |
| To explore the efficacy of Beauveria bassiana SW21, B.brongniartii SW22, Bacillus thuringiensis SW12 and Metarhizium anisopliae SW23 against Maladera orientalis, a pest of Camphora longepaniculata, this study measured the mortality rate, feeding amount, and the activities of protective enzymes (POD, SOD and CAT), detoxifying enzymes (AChE, CES, MFO, CYP450 and GST), and digestive enzymes (AMS, trypsin and lipase) in the gut of M.orientalis after treated with microbial agents. The results showed that the effectiveness of the microbial agents in increasing the mortality rate and reducing feeding amount of M.orientalis were followed the order: B.brongniartii SW22>Metarhizium anisopliae SW23>B.bassiana SW21>Bacillus thuringiensis SW12. All four microbial agents inhibited the activities of protective enzymes (SOD and CAT) while enhancing the activities of metabolic enzymes, including POD, AChE, CES, CYP450, GST, MFO, AMS, lipase, and trypsin, to varying degrees. The findings suggest that the microbial agents can suppress SOD and CAT activities, reducing the ability of M.orientalis to scavenge reactive oxygen species, leading to oxidative damage and increased mortality. Conversely, M.orientalis can compensate by upregulating the activities of other metabolic enzymes to maintain physiological functions. These microbial agents demonstrate potential for the green management of M.orientalis. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
