| 丁明碧1, 2, 王永芬2, 3*, 吴 丽1, 2, 余宏伟1, 2, 番华彩2, 曾 莉2, 徐胜涛2, 郑泗军2, 4*.罹病香蕉假茎象甲苏格兰白僵菌的分离鉴定及其生物学特性[J].植物保护,2025,51(2):82-96. |
| 罹病香蕉假茎象甲苏格兰白僵菌的分离鉴定及其生物学特性 |
| Isolation, identification and biological characteristics of Beauveria caledonica derived from diseased Odoiporus longicollis |
| 投稿时间：2024-04-24 修订日期：2024-06-13 |
| DOI：10.16688/j.zwbh.2024235 |
| 中文关键词: 香蕉假茎象甲 苏格兰白僵菌 生物学特性 致病力 |
| 英文关键词:Odoiporus longicollis Beauveria caledonica biological characteristics virulence |
| 基金项目:国家香蕉产业技术体系(CARS-31); 云南省万人计划“云岭学者”项目(YNWR-YLXZ-2018-018); 国际原子能机构项目(D23033-26673) |
| 作者 | 单位 | E-mail | | 丁明碧1, 2, 王永芬2, 3*, 吴 丽1, 2, 余宏伟1, 2, 番华彩2, 曾 莉2, 徐胜涛2, 郑泗军2, 4* | 1. 云南大学资源植物研究院, 昆明 650500
2. 云南省农业跨境有害生物绿色防控重点实验室, 云南省农业科学院农业环境资源研究所, 昆明 650205
3. 云南省农业科学院热带亚热带经济作物研究所, 保山 678000
4. 国际生物多样性中心, 昆明 650205 | 王永芬ynwyfmm@163.com; 郑泗军sijunzheng63@163.com |
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| 中文摘要: |
| 对云南省西双版纳州和昆明市东川区罹病的香蕉假茎象甲Odoiporus longicollis进行了病原分离。通过形态学鉴定及rDNA-ITS序列比对, 确定分离到的4个菌株均为苏格兰白僵菌Beauveria caledonica。通过对这4个分离株的离体培养条件探索及毒力测定, 表明离体培养的最佳条件为光周期L∥D=24 h∥0 h、温度25℃、在加有虫粉的PDA培养基上培养18 d, 此条件下的4个菌株菌落直径和产孢量最高, 其中Cs-1达到(65.17±0.74) mm和1.01×108个/cm2, 乳糖和牛肉膏分别为最适碳、氮源。生物测定结果表明, Cs-1菌株在接种浓度为1×109个/mL时对香蕉假茎象甲致死中时(LT50)最短, 为9.254 d, 致死中浓度(LC50)为3.618×106个/mL, 20 d后的致死率为86.67%。本研究首次在云南蕉园发现并鉴定的4株昆虫病原真菌均为苏格兰白僵菌, Cs-1菌株对香蕉假茎象甲具有较好的生防潜力, 可望今后应用于香蕉假茎象甲的生物防治。 |
| 英文摘要: |
| Four fungal strains were isolated from infected Odoiporus longicollis collected in Jinghong city, Xishuangbanna prefecture and Dongchuan district in Kunming city, Yunnan province. They were identified as Beauveria caledonica based on morphological characteristics and rDNA-ITS sequence analysis. The biological characteristics of the strains under different conditions (culture temperature, light duration, medium composition, pH, carbon and nitrogen sources) and their pathogenicity to O. longicollis were investigated. The results showed that 25℃, L∥D=24 h∥0 h, 18 days of culture on PDA medium supplemented with insect meal were optimal cultured condition with the maximum colony diameter and spore production of (65.17±0.74) mm and 1.01×108 spores/cm2, respectively, for Cs-1. The most suitable carbon and nitrogen sources were lactose and beef paste. Bioassays revealed that the Cs-1 strain, at an inoculation concentration of 1×109 spores/mL, achieved an LT50 of 9.254 days, an LC50 of 3.618×106 spores/mL, and a cumulative mortality rate of 86.67% after 20 days. This study marks the first identification of B. caledonica strains in banana plantations in Yunnan. The Cs-1 strain demonstrates significant potential as a biocontrol agent against O. longicollis and warrants further investigation for field application. |
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