| 危 潇2#, 黄大野1#, 姚经武1, 袁勤峰1, 汪汉成4, 黎妍妍3*, 曹春霞1*.基于转录组研究哈茨木霉WF2诱导的烟草抗病分子机制[J].植物保护,2025,51(2):44-51. |
| 基于转录组研究哈茨木霉WF2诱导的烟草抗病分子机制 |
| Transcriptome-based study on the molecular mechanism of Trichoderma harzianum WF2-induced disease resistance in tobacco |
| 投稿时间：2024-04-03 修订日期：2024-05-08 |
| DOI：10.16688/j.zwbh.2024183 |
| 中文关键词: 哈茨木霉 转录组分析 烟草 |
| 英文关键词:Trichoderma harzianum transcriptome analysis tobacco |
| 基金项目:湖北省烟草公司科技项目(027Y2022-005); 中国烟草总公司重大科技项目［110202201019(LS-03), 110202101048(LS-08), 110202001035(LS-04)］; 湖北省农业科技创新中心创新团队项目(2021-620-000-001-027) |
| 作者 | 单位 | E-mail | | 危 潇2#, 黄大野1#, 姚经武1, 袁勤峰1, 汪汉成4, 黎妍妍3*, 曹春霞1* | 1. 湖北省生物农药工程研究中心, 国家生物农药工程技术研究中心, 武汉 430064
2. 湖北工业大学, 武汉 430068
3. 湖北省烟草科学研究院, 武汉 430030
4. 贵州省烟草科学研究院, 贵阳 550081 | 黎妍妍yanyanli0025@126.com; 曹春霞cao-chunxia@163.com |
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| 中文摘要: |
| 为了深入探究哈茨木霉WF2诱导烟草抗病性的分子机制, 利用转录组测序技术与荧光定量PCR技术, 将经过哈茨木霉WF2处理的烟苗与对照的转录组进行对比与分析。结果发现, WF2处理共诱导了377个差异表达基因(DEGs), 其中341个DEGs上调表达, 36个DEGs下调表达。通过对差异表达基因进行GO和KEGG富集分析, 发现WF2主要诱导乙烯(ET)信号通路和MAPK信号通路相关基因的上调表达, 其中包括大量植物防御抗性相关基因, 如编码ERF、WRKY、TGA等转录因子的基因。此外, 哈茨木霉WF2还诱导了非生物胁迫相关的信号调节因子如DREB、CRK、PYL等的上调表达。 |
| 英文摘要: |
| To further explore the molecular mechanism of Trichoderma harzianum WF2-induced tobacco disease resistance, transcriptome sequencing technology and fluorescence quantitative PCR technology were used to compare and analyze transcriptional level changes between T.harzianum-treated and control groups. The results showed that WF2 treatment induced a total of 377 differentially expressed genes (DEGs), including 341 up-regulated DEGs and 36 down-regulated DEGs. GO and KEGG enrichment analyses revealed that T.harzianum WF2 mainly induced the up-regulated expression of genes related to ethylene (ET) signaling pathway and MAPK signaling pathway, which included numerous plant defense-related genes encoding transcription factors such as ERF, WRKY, and TGA. In addition, T.harzianum WF2 also induced the up-regulation of abiotic stress-related signal regulators like DREB, CRK and PYL. |
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