| 苗治国1, 洪 牛1, 刘东阳2, 徐蓬军3, 邓 全2, 闫芳芳4, 余佳敏5*, 李茂业1, 刘 苏1*.桃蚜几丁质合成酶1基因的分子特征及其生物学功能分析[J].植物保护,2025,51(2):35-43. |
| 桃蚜几丁质合成酶1基因的分子特征及其生物学功能分析 |
| Molecular characterization and functional analysis of the chitin synthase 1 gene from Myzus persicae |
| 投稿时间：2024-03-22 修订日期：2024-04-30 |
| DOI：10.16688/j.zwbh.2024162 |
| 中文关键词: 桃蚜 几丁质合成酶 序列分析 表达模式 RNA干扰 |
| 英文关键词:Myzus persicae chitin synthase sequence analysis expression pattern RNA interference |
| 基金项目:安徽省高等学校科学研究项目(2023AH051053); 安徽省教育厅优秀青年人才支持计划(gxyq2021167); 中国烟草总公司四川省公司科技项目(SCYC202410, SCYC202112); 烟草行业青年科技托举人才项目(YCQTSC202401); 烟草行业烟草病虫害监测与综合治理重点实验室开放课题(KLTPMIMT2023-09) |
| 作者 | 单位 | E-mail | | 苗治国1, 洪 牛1, 刘东阳2, 徐蓬军3, 邓 全2, 闫芳芳4, 余佳敏5*, 李茂业1, 刘 苏1* | 1. 安徽农业大学植物保护学院, 农产品质量与生物安全教育部重点实验室, 合肥 230036
2. 四川省烟草公司凉山州公司, 西昌 615000
3. 中国农业科学院烟草研究所, 青岛 266101
4. 四川省烟草公司攀枝花市公司, 攀枝花 617099
5. 中国烟草总公司四川省公司, 四川省烟草科学研究所, 成都 610041 | 余佳敏scycyujm@163.com; 刘苏suliu@ahau.edu.cn |
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| 中文摘要: |
| 桃蚜Myzus persicae是重要的农业害虫。本研究分析了桃蚜几丁质合成酶1 (chitin synthase 1, CHS1)基因MperCHS1的序列特征, 通过实时荧光定量PCR测定了MperCHS1基因在不同发育阶段的表达模式, 利用病毒介导的基因沉默(virus-induced gene silencing, VIGS)技术解析了MperCHS1的生物学功能。结果表明, MperCHS1含有19个外显子, 开放阅读框为4 707 bp, 其编码的蛋白具有保守的几丁质合成酶结构域, 且与豌豆蚜CHS1亲缘关系最近。MperCHS1在1龄若虫期表达量最低, 而在2龄和3龄若虫期表达量最高。沉默MperCHS1后48、72、96 h和120 h, 桃蚜存活率分别下降至68.6%、57.1%、50.8%和49.2%。此外, 沉默MperCHS1导致41.7%的若蚜无法正常蜕皮, 雌成蚜的产蚜量也显著减少。本论文明确了MperCHS1在桃蚜生长发育和生殖中的重要功能, 研究结果为开发基于MperCHS1基因沉默的桃蚜新型防治技术奠定了基础。 |
| 英文摘要: |
| The green peach aphid, Myzus persicae, is an important agricultural insect pest. This study analyzed the molecular characteristics of the chitin synthase 1 gene (MperCHS1) in M. persicae. The expression pattern of MperCHS1 during different developmental stages was detected using real-time quantitative PCR. The biological function of MperCHS1 was explored via the virus-induced gene silencing (VIGS) technology. The results showed that the MperCHS1 gene consisted of 19 exons and contained an open reading frame of 4 707 bp. The protein encoded by MperCHS1 had a conserved chitin synthase domain and is most closely related to CHS1 in the pea aphid, Acyrthosiphon pisum. The lowest expression level of MperCHS1 was observed in first-instar nymphs, while the highest expression occurred during second- and third-instar nymphal stages. Following MperCHS1 silencing, the survival rate of M. persicae decreased to 68.6%, 57.1%, 50.8% and 49.2% at 48, 72, 96 h, and 120 h post-treatment, respectively. Additionally, 41.7% of nymphs exhibited an incomplete molting phenotype, and the reproductive output of female adults was significantly reduced. This study highlights the critical roles of MperCHS1 in the growth, development and reproduction of M. persicae, providing a foundation for developing novel pest control strategies targeting M. persicae. |
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