| 刘晨浩1, 杨明寰1, 王 齐1, 严善春1*, 刘 伟2*, 王桂荣2.交配前后雌性橘小实蝇触角和脑部组织比较转录组分析[J].植物保护,2025,51(2):18-34. |
| 交配前后雌性橘小实蝇触角和脑部组织比较转录组分析 |
| Comparative transcriptome analysis of the antennal and brain tissues of female Bactrocera dorsalis before and after mating |
| 投稿时间：2024-03-02 修订日期：2024-05-12 |
| DOI：10.16688/j.zwbh.2024109 |
| 中文关键词: 橘小实蝇 交配行为 触角 脑 转录组 |
| 英文关键词:Bactrocera dorsalis mating behavior antennae brain transcriptome |
| 基金项目:深圳市科技计划(KQTD20180411143628272); 深圳市大鹏新区科技创新和产业发展专项(PT202101-02); 国家重点研发计划(2022YFD1700200) |
| 作者 | 单位 | E-mail | | 刘晨浩1, 杨明寰1, 王 齐1, 严善春1*, 刘 伟2*, 王桂荣2 | 1. 东北林业大学林学院, 森林生态系统可持续经营教育部重点实验室, 哈尔滨 150040
2. 中国农业科学院(深圳)农业基因组研究所, 岭南现代农业科学与技术广东省实验室深圳分中心, 农业农村部合成生物重点实验室, 深圳 518120 | 严善春yanshanchun@126.com; 刘伟liuwei11@caas.cn |
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| 中文摘要: |
| 橘小实蝇Bactrocera dorsalis是我国水果产业的重要害虫, 雌虫初次交配后再次交配的行为有明显变化, 这种变化可能受神经系统调节。本研究通过行为学试验观察12日龄的橘小实蝇成虫初次交配后连续4 d的再交配行为; 利用高通量测序技术分别对交配前后橘小实蝇雌虫的触角及脑组织进行转录组测序, 分析交配前后差异表达的基因; 以P＜10-4为标准筛选交配后雌虫脑组织中与交配行为变化相关的候选基因, 并利用qPCR对候选基因转录水平进行验证; 利用生物信息学方法在全基因组中鉴定候选基因的基因家族成员并分析其在外周神经组织中的表达模式。结果表明, 初次交配后连续4 d橘小实蝇雄虫交配率均在94%以上, 而雌虫在未产卵的情况下交配率均低于11%; 与未交配状态相比, 交配后橘小实蝇雌虫触角组织中有1个基因的转录显著上调, 3个基因显著下调; 脑组织中有20个基因显著上调, 22个基因显著下调。从交配后雌虫脑组织中20个上调基因中共筛选到5个候选差异基因, 其中2个属于卵黄原蛋白(vitellogenin, Vg)基因家族, 3个属于类蜕皮素激酶(ecdysteroid kinase-like, EcKL)基因家族; 橘小实蝇全基因组中共鉴定到59个EcKL基因家族成员和11个Vg基因家族成员, 其中49个EcKL和9个Vg在脑和外周神经组织中有表达。本研究结合行为学试验及组学数据对交配后橘小实蝇雌虫触角及脑组织中转录组进行了分析, 并分析了与交配后再次交配行为相关的候选基因的基因家族成员和表达模式, 为进一步研究调控橘小实蝇交配行为的关键基因及开发相关防控技术提供了基础。 |
| 英文摘要: |
| Bactrocera dorsalis is a major pest in China’s fruit industry. The female’s mating behavior shows distinct changes after the first mating, which may be linked to the nervous system. This study observed the remating behavior of 12-day-old B. dorsalis adults for four consecutive days after their first mating. Transcriptome sequencing of antennal and brain tissues was conducted to identify differentially expressed genes before and after mating. A threshold of P＜10-4 was set to screen potential candidate genes associated with post-mating behavioral changes, and qPCR was employed to validate expression changes. Bioinformatics methods were used to identify gene family members of candidate genes and analyze their expression patterns in peripheral nervous tissues. The results showed that within four days after the initial mating, the remating rate of male B. dorsalis was above 94%, while that of females was below 11% if they had not laid eggs. In comparison to the unmated state, one gene was significantly up-regulated and three were significantly down-regulated in the female antennae after mating, while 20 genes were significantly up-regulated and 22 genes were significantly down-regulated in the female brain tissue. Five candidate genes were identified, including two from the vitellogenin (Vg) gene family and three from the ecdysteroid kinase-like (EcKL) gene family. In the genome, 59 EcKL and 11 Vg family members were identified, with 49 EcKL and nine Vg genes expressed in the brain and peripheral nervous tissues. This study integrated behavioral experiments and transcriptomic data to examine differentially expressed genes in the brain and antennal tissues of female B. dorsalis post-mating, laying a foundation for further exploration of key genes that regulate the mating behavior of B. dorsalis and the development of relevant prevention and control technologies. |
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