| 王道涵1, 瞿红叶1, 米仁军2, 谈家金1*.灰葡萄孢培养基种类和继代培养对松材线虫致病力的影响[J].植物保护,2024,50(6):106-111. |
| 灰葡萄孢培养基种类和继代培养对松材线虫致病力的影响 |
| Influence of Botrytis cinerea culture mediums and subculturing on the virulence of Bursaphelenchus xylophilus |
| 投稿时间：2024-03-06 修订日期：2024-04-03 |
| DOI：10.16688/j.zwbh.2024121 |
| 中文关键词: 松材线虫 繁殖力 致病力 培养基 继代培养 |
| 英文关键词:Bursaphelenchus xylophilus fecundity virulence culture medium subculturing |
| 基金项目:国家重点研发计划（2021YFD1400900） |
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| 中文摘要: |
| 本试验在8种培养基上培养灰葡萄孢Botrytis cinerea,以灰葡萄孢喂饲松材线虫Bursaphelenchus xylophilus，采用3年生黑松Pinus thunbergii作为接种材料,研究松材线虫繁殖力和致病力的变化,同时探究了在PDA-灰葡萄孢上继代培养84代的松材线虫致病力的变化。结果表明,以玉米、小麦和PDA 3种培养基培养的灰葡萄孢喂饲松材线虫,其繁殖力显著高于CDA和4种合成培养基,其中PDA-灰葡萄孢上的松材线虫数量最多,比合成培养基C或D培养的灰葡萄孢上的线虫数量提升了48.94%;接种PDA-灰葡萄孢组松材线虫的黑松病情指数最高,较最低的合成培养基D-灰葡萄孢组高了47.87%。松材线虫在PDA-灰葡萄孢上继代培养至第14代后,继代次数的增加会导致其致病力下降,接种第79代松材线虫后黑松病情指数已为0。因此,为保证松材线虫的致病力不衰退,应使用PDA-灰葡萄孢培养基喂饲松材线虫,且松材线虫继代培养应控制在14代以内。研究结果将为松材线虫的繁殖技术与致病相关机理分析等提供参考。 |
| 英文摘要: |
| In this study, changes in the fecundity and virulence of Bursaphelenchus xylophilus fed on Botrytis cinerea growing on eight different mediums and changes in the virulence of B. xylophilus subcultured on PDA for 84 generations were examined, using 3-year-old Pinus thunbergii as the inoculum material. The results showed that the fecundity of B. xylophilus fed on B. cinerea growing on three mediums, namely corn, wheat, and PDA, was significantly higher than those fed on B. cinerea on CDA and four synthetic mediums. The population size of B. xylophilus was highest on the combination of PDA and B. cinerea, 48.94% higher than those the combination of synthetic medium C/D and B. cinerea. The disease index of P. thunbergii inoculated with B. xylophilus fed on B. cinerea on PDA was also the highest, 47.87% higher than inoculation with B. xylophilus fed on B. cinerea on the synthetic medium D. However, after 14 generations of subculturing B. xylophilus fed on B. cinerea on PDA, an increase in successive generations led to a decrease in virulence. By the 79th generation, the disease index of P. thunbergii inoculated with B. xylophilus was zero. Therefore, to maintain virulence, B. xylophilus should be cultured on B. cinerea growing on PDA and subcultured for no more than 14 generations. These findings provide valuable insights into the reproduction technology and pathogenic mechanisms of B. xylophilus. |
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