| 李 智1,2, 李京晶2, 席先梅3, 田小卫1*, 刘 倩2*.内蒙古兴和县腐烂茎线虫群体的分离和鉴定[J].植物保护,2024,50(6):77-84. |
| 内蒙古兴和县腐烂茎线虫群体的分离和鉴定 |
| Isolation and identification of Ditylenchus destructor from Xinghe county, Inner Mongolia |
| 投稿时间：2024-03-20 修订日期：2024-05-13 |
| DOI：10.16688/j.zwbh.2024157 |
| 中文关键词: 马铃薯 腐烂茎线虫 形态特征 单倍型 |
| 英文关键词:potato Ditylenchus destructor morphological character haplotype |
| 基金项目:国家重点研发计划（2023YFD1400400） |
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| 中文摘要: |
| 内蒙古是我国重要的马铃薯种植区,明确马铃薯线虫病的病原,对种植区内的线虫检疫和防治具有重大意义。本研究从兴和县采集出现空心、干腐等症状的马铃薯,分离线虫后进行形态学鉴定、分子生物学鉴定。结果表明,兴和县线虫群体的形态特征与腐烂茎线虫Ditylenchus destructor相似,形态测量值虽存在差异但范围值基本一致;其rDNA-ITS序列与腐烂茎线虫序列相似性为100%;系统发育树结果显示,供试群体与腐烂茎线虫C型、D型群体聚为一支,其序列和腐烂茎线虫C型的序列最大相似性达99.87%，和D型序列的相似性为99.26%。综上,将兴和县线虫群体鉴定为腐烂茎线虫C型群体。致病性测定表明，分离线虫接种马铃薯后引起的症状与自然发病症状一致。该研究结果为兴和县制定马铃薯腐烂茎线虫防治策略提供了理论依据。 |
| 英文摘要: |
| Inner Mongolia is one of the most important potato planting area in China. Identifying the pathogen responsible for potato nematode diseases is crucial for quarantine and control in this region. Potatoes with symptoms of hollow and dry rot from Xinghe county were collected, the nematodes were separated from these potatoes,and a comprehensive analysis, including morphological identification, molecular biological identification were performed. The results showed that the nematodes share similar morphological characteristics with Ditylenchus destructor. Although there were some differences in morphological measurements, the range values were generally consistent. The rDNA-ITS sequences of these nematodes showed 100% similarity to D. destructor. Phylogenetic analysis showed that the tested population clustered into the same branch with the C-type and D-type, with sequence similarities of 99.87% and 99.26%, respectively. The symptoms caused by nematode isolates are consistent with those in the field. In summary, the nematode population in Xinghe county was identified as the C-type of D. destructor. The results providing a theoretical basis for developing control strategies for D. destructor in Xinghe county. |
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