| 陈宗雄, 杨紫薇, 惠仁杰, 丁晓帆*.丁香酚对南方根结线虫活性测定方法的比较[J].植物保护,2024,50(5):219-223. |
| 丁香酚对南方根结线虫活性测定方法的比较 |
| Comparison of three bioassay methods of eugenol against Meloidogyne incognita |
| 投稿时间：2023-09-25 修订日期：2023-11-09 |
| DOI：10.16688/j.zwbh.2023495 |
| 中文关键词: 丁香酚 南方根结线虫 生物测定方法 |
| 英文关键词:eugenol Meloidogyne incognita bioassay method |
| 基金项目:海南大学教改项目（hdjy2163） |
|
| 摘要点击次数: 699 |
| 全文下载次数: 608 |
| 中文摘要: |
| 为了准确评价丁香酚(C10H12O2)对南方根结线虫Meloidogyne incognita的毒杀效果, 本研究采用水琼脂平板熏蒸法、土壤熏蒸法和浸虫法分别测定了丁香酚对南方根结线虫2龄幼虫(J2)和卵的毒力, 比较3种测定方法的测定效果。结果表明, 水琼脂平板熏蒸法、土壤熏蒸法和浸虫法测得的丁香酚对2龄幼虫的LC50分别为123.5、140.2 μL/L和309.7 μL/L; 丁香酚浓度为50 μL/L时, 水琼脂平板熏蒸法测得的卵孵化抑制率高达97.10%, 而土壤熏蒸法和浸虫法测得的卵孵化抑制率仅分别为32.79%、11.50%; 同时, 水琼脂平板可作为线虫或卵的可视化载体, 便于在体视显微镜下观察拍照。综上, 水琼脂平板熏蒸法具有药效发挥更充分、可视化、操作简便等优点, 该方法更适用于熏蒸类化合物的杀线虫生物测定。 |
| 英文摘要: |
| In order to accurately evaluate the toxicity of eugenol (C10H12O2) on Meloidogyne incognita, the second-stage juveniles (J2) and eggs of M.incognita were treated with eugenol by water agar plate fumigation, soil fumigation, and immersion, respectively. The results showed that the LC50 value of eugenol by water agar plate fumigation was 123.5 μL/L, while those by soil fumigation and immersion were 140.2 μL/L and 309.7 μL/L, respectively. The inhibition rate of egg hatching by 50 μL/L eugenol with water agar plate fumigation was 97.10%, while those by soil fumigation and immersion method were only 32.79% and 11.50%, respectively. Meanwhile, the water agar plate could be used as a visual carrier of J2 or eggs under stereomicroscopy for easy observation. In summary, the water agar plate fumigation has the advantages of fully exerting virulence effect of eugenol, visualization, and simple operation. It is more suitable for the nematode bioassay of fumigation compounds. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
