| 步洪松1, 2, 王 丁2, 张馨月2, 周 丹2, 郑生文3, 匡云波2, 叶祖云2*.4种方法脱除太子参BBWV和TuMV病毒的效果比较[J].植物保护,2024,50(5):213-218. |
| 4种方法脱除太子参BBWV和TuMV病毒的效果比较 |
| Comparision of effect of four methods for the virus-free of BBWV and TuMV viruses in Pseudostellaria heterophylla |
| 投稿时间：2023-10-30 修订日期：2024-01-24 |
| DOI：10.16688/j.zwbh.2023562 |
| 中文关键词: 太子参 花叶病毒 脱病毒方法 病毒检测 |
| 英文关键词:Pseudostellaria heterophylla mosaic virus virus-free method virus detection |
| 基金项目:中央引导地方科技发展专项资金项目（202113030）；宁德师范学院科研项目（2020FZ07, 2021FZ03, 2022ZX01）；福建省特色药用植物工程技术研究中心学生科研项目（PC202101, PC202104） |
| 作者 | 单位 | E-mail | | 步洪松1, 2, 王 丁2, 张馨月2, 周 丹2, 郑生文3, 匡云波2, 叶祖云2* | 1. 福建农林大学生命科学学院, 福州 350002
2. 福建省特色药用植物工程技术研究中心, 宁德师范学院生命科学学院, 宁德 352100
3. 福建柘参生物科技研究股份有限公司, 宁德 355300 | zyye0593@163.com |
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| 中文摘要: |
| 以太子参‘柘参1号’品种为试验材料, 比较微茎尖培养、低温处理(2~4℃)结合微茎尖培养、超低温(-196℃)培养、种胚培养等4种方法对蚕豆萎蔫病毒(broad bean wilt virus, BBWV)和芜菁花叶病毒(turnip mosaic virus, TuMV)的脱除效果, 结果显示采用低温处理结合微茎尖培养, 太子参成活率为64.7%, BBWV和TuMV的脱毒率分别为63.6%和31.8%; 采用种胚培养的成活率为56.7%, 对BBWV和TuMV的脱毒率均可达100%。病毒TuMV相较BBWV更难脱除。 |
| 英文摘要: |
| The effects of four methods, including microstem tip culture, low temperature (2－4℃) treatment combined with microstem tip culture, ultra-low temperature (-196℃) culture and embryo culture, on the virus-free of broad bean wilt virus (BBWV) and turnip mosaic virus (TuMV) from Pseudostellaria heterophylla ‘Zheshen No.1’ were compared. The results showed that the survival rate of P. heterophylla was 64.7%, and the virus-free rates of BBWV and TuMV were 63.6% and 31.8%, respectively, using low temperature treatment combined with micro-stem tip culture. The survival rate of embryo culture was 56.7%, and the virus-free rate of BBWV and TuMV was 100%. P. heterophylla mosaic virus TuMV is more difficult to remove than BBWV. |
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