| 王芹芹1, 冯靖文1, 刘 迪1, 张国彦2, 王 立1, 毛雪伟1, 汪梅子1, 崔凯娣1, 周 琳1*.松油醇与d-柠檬烯对花生白绢病菌的生物活性及协同增效作用[J].植物保护,2024,50(5):112-119. |
| 松油醇与d-柠檬烯对花生白绢病菌的生物活性及协同增效作用 |
| Biological activity and synergistic effects of terpineol and d-limonene against Sclerotium rolfsii, the causal agent of peanut southern blight |
| 投稿时间：2023-10-20 修订日期：2023-12-02 |
| DOI：10.16688/j.zwbh.2023543 |
| 中文关键词: 松油醇 d-柠檬烯 花生 病原真菌 齐整小核菌 |
| 英文关键词:terpineol d-limonene peanut pathogenic fungi Sclerotium rolfsii |
| 基金项目:河南省重大科技专项(221100110100)；河南省花生产业技术体系植保岗位专家（S2012-05-G06）；河南农业大学高层次人才专项支持基金(30501425) |
| 作者 | 单位 | E-mail | | 王芹芹1, 冯靖文1, 刘 迪1, 张国彦2, 王 立1, 毛雪伟1, 汪梅子1, 崔凯娣1, 周 琳1* | 1. 河南农业大学植物保护学院, 河南省新型农药创制与应用重点实验室, 河南省绿色农药创制工程技术研究中心, 郑州 450046
2. 河南省植物保护检疫站, 郑州 450002 | zhoulinhenau@163.com |
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| 中文摘要: |
| 为明确植物源松油醇和d-柠檬烯对花生白绢病菌的生物活性和应用潜力, 采用菌丝生长速率法测定了松油醇与d-柠檬烯对花生白绢病菌的抑菌活性, 室内筛选了对花生白绢病菌抑制效果好的最佳混配比(质量比), 并采用共毒系数法和盆栽法评价了二者的协同增效作用。结果表明, 松油醇与d-柠檬烯对花生白绢病菌的抑制活性均较强, 抑制中浓度(EC50)分别为49.23 μg/mL和60.87 μg/mL。且在500 μg/mL和1 000 μg/mL浓度下, 松油醇处理15 d和30 d可完全抑制花生白绢病菌菌丝生长, 菌核形成以及菌核再萌发。而相同条件下d-柠檬烯对菌丝生长、菌核形成和菌核萌发无影响。在特定浓度下, 松油醇与d-柠檬烯按不同质量比复配均能提高对花生白绢病菌的抑制作用, 其中松油醇和d-柠檬烯以1∶4和1∶2复配时增效作用最为显著, 共毒系数(CTC)分别为147.87和216.57。盆栽试验结果显示, 松油醇和d-柠檬烯以1∶4和1∶2复配的防效均高于单剂。综上, 松油醇和D-柠檬烯复配可作为新型植物源杀菌剂进行开发利用。 |
| 英文摘要: |
| To clarify the biological activity and application potential of terpineol and d-limonene from plant essential oils in fighting peanut southern blight caused by Sclerotium rolfsii, their inhibitory activities were tested using the mycelial growth rate method. The optimal mixing mass ratio of terpineol and d-limonene for controlling S. rolfsii was screened in the laboratory, and the synergistic effect was evaluated using co-toxicity coefficient method and pot experiments. The results showed that the strongest inhibitory activity of terpineol and d-limonene against S. rolfsii was achieved with EC50 values of 49.23 μg/mL and 60.87 μg/mL, respectively. At 500 μg/mL and 1 000 μg/mL, terpineol completely inhibited mycelial growth, sclerotium formation and re-germination when treatment lasted for 15 and 30 days. However, under same treatment conditions, d-limonene did not inhibit mycelial growth for a longer period and did not inhibit sclerotium formation and re-germination. Compared with the single agent, the inhibition rates of combination of terpineol and d-limonene against S. rolfsii at specific concentrations with different mass ratios were elevated. The synergistic effect of terpineol combined with d-limonene in mass ratios of 1∶4 and 1∶2 was the most significant, with co-toxicity coefficients of 147.87 and 216.57, respectively. Potted experiments showed that the control effect of the combination of terpineol and d-limonene in mass ratios of 1∶4 and 1∶2 was higher than that of a single agent, indicating that this combination can be developed and utilized as a new plant-derived fungicide. |
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