| 陈晶伟1, 2, 黄泓晶1, 李 硕1, 吴华香1, 程 曦1, 肖 顺1, 刘国坤1*.寄生番茄的拟禾本科根结线虫的鉴定及对番茄不同品种的趋性[J].植物保护,2024,50(5):63-71. |
| 寄生番茄的拟禾本科根结线虫的鉴定及对番茄不同品种的趋性 |
| Identification of Meloidogyne graminicola on tomato and it’s taxis to the different tomato cultivars |
| 投稿时间：2023-10-25 修订日期：2023-12-21 |
| DOI：10.16688/j.zwbh.2023532 |
| 中文关键词: 番茄 拟禾本科根结线虫 鉴定 趋性 致病性 |
| 英文关键词:Solanum lycopersicum Meloidogyne graminicola identification taxis pathogenicity |
| 基金项目:福建省现代农业（水稻）产业技术体系建设项目（闽财教指〔2021〕61号） |
| 作者 | 单位 | E-mail | | 陈晶伟1, 2, 黄泓晶1, 李 硕1, 吴华香1, 程 曦1, 肖 顺1, 刘国坤1* | 1. 福建农林大学, 生物农药与化学生物学教育部重点实验室, 福州 350002
2. 江苏徐淮地区徐州农业科学研究所, 农业农村部甘薯生物学与遗传育种重点实验室, 徐州 221131 | liuguok@ 126.com |
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| 中文摘要: |
| 2019年福建省莆田市番茄‘农科180’品种发生严重的根结线虫病, 根尖处根结多呈“靴”状。本研究通过形态学、线虫rDNA-ITS、28S rDNA D2-D3序列分析与特异性引物检测, 明确病原线虫种群为拟禾本科根结线虫Meloidogyne graminicola。这是在福建省番茄上首次报道拟禾本科线虫危害番茄。为探讨不同番茄品种对拟禾本科根结线虫的感、抗性, 在Pluronic F-127胶体介质中测定该种群2龄幼虫(J2)对5个番茄品种(农科180’ ‘仙客1号’ ‘仙客6号’ ‘红美人’和‘硬粉8号’)根尖的趋性, 结果表明, 趋向于‘农科180’的J2数量显著大于其他4个番茄品种, 品种有无Mi基因与J2趋性无关; 室内致病性试验表明, 接种后35 d调查, ‘农科180’发病率为100%, 病情指数为60; ‘仙客1号’ ‘仙客6号’ ‘红美人’和‘硬粉8号’的发病率分别为53%、40%、33%和27%, 病情指数均低于11。研究表明, 番茄‘农科180’根尖对J2具有强烈的吸引性是其感病的原因之一。生产中应重视番茄品种对拟禾本科根结线虫的感抗性差异。 |
| 英文摘要: |
| Serious root-knot nematode disease of tomato (Solanum lycopersicum cv. ‘Nongke 180’) with typically hooked-shaped galls at the root tip was found in the Putian region, Fujian province. Based on the morphological and molecular analyses of rDNA-ITS and 28S rDNA D2-D3 sequences, and detection using species-specific primers, the nematode was identified as Meloidogyne graminicola. This is the first report of a natural infection of M. graminicola on tomato in Fujian province. Taxis assays of second stage juveniles (J2s) of M. graminicola to root tips of five tomato cultivars, including ‘Nongke 180’ ‘Xianke 1’ ‘Xianke 6’ ‘Hongmeiren’ and ‘Yingfen 8’ were tested using the Pluronic F-127 gel system. Greater numbers of J2s were significantly attracted to ‘Nongke 180’ than to the other four cultivars. There no association existed between attractive ability and the presence of Mi-resistance gene in the five cultivars. Pathogenicity of the nematode to the five tomato cultivars was conducted and compared, the results showed that the incidence of ‘Nongke 180’ was 100% incidence with a disease index of 60, while the incidence of ‘Xianke 1’ ‘Xianke 6’ ‘Hongmeiren’ and ‘Yingfen 8’ were 53%, 40%, 33%, and 27%, respectively, with all disease indexes being less than 11. The research showed that tomato cv. ‘Nongke 180’ was a susceptible host for M. graminicola, with strong attraction to J2s of the nematode. |
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