| 单佳祁1#, 刘保军1#, 张李娅1, 王 澍1, 李克梅1*, 白剑宇2*.2个分离自新疆不同枣树样本的花叶伴随病毒基因组测定[J].植物保护,2024,50(4):108-117. |
| 2个分离自新疆不同枣树样本的花叶伴随病毒基因组测定 |
| Complete nucleotide sequences of the genomes of two varietal strains of jujube mosaic-associated virus in Xinjiang |
| 投稿时间：2023-09-08 修订日期：2023-11-13 |
| DOI：10.16688/j.zwbh.2023470 |
| 中文关键词: 高通量测序 枣树 枣树花叶伴随病毒 全核苷酸序列 |
| 英文关键词:high-throughput sequencing jujube jujube mosaic-associated virus complete nucleotide sequence |
| 基金项目:国家林业和草原局林业科技专项（65000022P00789110010X） |
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| 中文摘要: |
| 近几年来, 新疆南部红枣各种植区大面积发生疑似枣树花叶伴随病毒病症状的病害, 造成枣树叶片出现花叶卷曲, 枣果畸形失去商品价值, 甚至大量落果, 造成绝收绝产。对于引起此病害的病原现在尚未完全明确。本文从新疆南部红枣种植区采集病叶, 通过高通量测序证实病叶中存在枣树花叶伴随病毒(jujube mosaic-associated virus, JuMaV), 将JuMaV的2个分离株AKS和YPH的全序列(登录号分别为MW892537和OL739568), 与已发表的枣树枣花叶伴随病毒(JuMaV-Z6)的全序列(登录号:KX852476.1)进行了比较, 结果显示, JuMaV-AKS和JuMaV-YPH与JuMaV-Z6的核苷酸一致性分别为99.54%和95.93%, 氨基酸一致性分别为100%和98.48%。JuMaV-AKS和JuMaV-YPH与JuMaV-Z6在反转录酶(RT)和RNA酶H (RNase H)编码区有较大差异, 表明枣树花叶伴随病毒在新疆的红枣上正在发生变异, 但还未成为新的种, 暂时统一称为枣树花叶伴随病毒XJ分离株(JuMaVXJ)。上述结果为新疆红枣花叶病病原的确认并为该病害的精准防控奠定了基础。 |
| 英文摘要: |
| In recent years, large areas of suspected jujube mosaic-associated virus causing symptoms have been observed in jujube planting areas in the southern Xinjiang, leading to jujube leaf curling, fruit losing their commercial value because of deformity, or fruit heavily falling, resulting in severe harvest and production losses. The pathogen of this disease remains unclear. High-throughput sequencing result confirmed the presence of jujube mosaic-associated virus (JuMaV) in the diseased leaves collected from jujube cultivation areas in southern Xinjiang in this study. The full sequences of two isolates, JuMaV-AKS, and JuMaV-YPH, were determined (accession numbers were MW892537 and OL739568, respectively), and compared with jujube mosaic-associated virus JuMaV-Z6 (accession no: KX852476.1) published in Genbank, the results showed that JuMaV-AKS and JuMaV-YPH shared 99.54% and 95.93% nucleotide identity with JuMaV-Z6, respectively, and 100% and 98.48% amino acid identity, respectively. Notably, JuMaV-AKS and JuMaV-YPH significantly differed from JuMaV-Z6 in the reverse transcriptase (RT) and RNA enzyme H (RNase H) coding region, suggesting ongoing mutation of jujube mosaic-associated virus in Xinjiang, although they have not yet evolved into a new species. Therefore, they were provisionally termed as jujube mosaic-associated virus XJ isolate (JuMaVXJ). The above results lay a foundation for the identification and precise control of the pathogen responsible for jujube mosaic disease in Xinjiang. |
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