| 杨 菲1, 2#, 张明振1#, 周雪平1*, 李方方1*.侵染中国河北大豆的大豆花叶病毒的鉴定和全基因组序列分析[J].植物保护,2024,50(4):39-47. |
| 侵染中国河北大豆的大豆花叶病毒的鉴定和全基因组序列分析 |
| Identification and complete genome sequence analysis of soybean mosaic virus from soybean in Hebei, China |
| 投稿时间：2023-06-28 修订日期：2023-09-23 |
| DOI：10.16688/j.zwbh.2023325 |
| 中文关键词: 大豆花叶病毒 小RNA深度测序 基因组序列 |
| 英文关键词:soybean mosaic virus (SMV) small RNA sequencing genome sequence |
| 基金项目:国家自然科学基金（31972244, 32172385） |
| 作者 | 单位 | E-mail | | 杨 菲1, 2#, 张明振1#, 周雪平1*, 李方方1* | 1. 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193
2. 河北省农林科学院植物保护研究所, 农业农村部华北北部作物有害生物综合治理重点实验室, 河北省农业有害生物综合防治技术创新中心, 河北省作物有害生物综合防治国际科技联合研究中心, 保定 071000 | 周雪平zzhou@zju.edu.cn;李方方lifangfang@caas.cn |
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| 中文摘要: |
| 本研究利用小RNA深度测序技术在河北卢龙大豆叶片上检测到6株大豆花叶病毒(soybean mosaic virus, SMV), 命名为SMV-Gm1~SMV-Gm6。根据小RNA深度测序结果和参考基因组序列设计引物克隆了SMV河北分离物的基因组序列。测序结果经拼接后获得了6个SMV基因组全长序列, 大小分别为9 588 nt(SMV-Gm1、SMV-Gm3和SMV-Gm5)和9 584 nt(SMV-Gm2、SMV-Gm4和SMV-Gm6)。开放阅读框位于基因组第132位至第9 332位核苷酸, 编码一个多聚蛋白(分子量约为350 kD)。BLAST比对和系统发育分析发现, SMV-Gm1、SMV-Gm3和SMV-Gm5与江苏SMV分离物(登录号:MH919386)的基因组核苷酸序列相似性最高, 为98.06%~98.07%, 且遗传距离较近, 并与江苏、浙江和山西SMV分离物聚为一小簇;SMV-Gm2、SMV-Gm4和SMV-Gm6与韩国SMV分离物(登录号:FJ640954)的基因组核苷酸序列相似性最高, 为98.48%~98.51%, 且遗传距离较近。 |
| 英文摘要: |
| In this study, six isolates of soybean mosaic virus (labelled SMV-Gm1 to SMV-Gm6) were detected from soybean in Lulong, Hebei province using small RNA deep sequencing technology. Based on the sequencing results and a reference genome sequence, specific primers were designed to clone the genome sequences of these SMV isolates. Following assembly, six full-length SMV genome sequences were obtained, with sizes of 9 588 nt for SMV-Gm1, SMV-Gm3, and SMV-Gm5, and 9 584 nt for SMV-Gm2, SMV-Gm4, and SMV-Gm6. The open reading frame is located between 132nd to 9 332nd nucleotides in each genome, encoding a polyprotein with a molecular weight of approximately 350 kD. BLAST alignment and phylogenetic analysis showed that SMV-Gm1, SMV-Gm3, and SMV-Gm5 showed the highest sequence similarity (98.06% to 98.07%) and relatively close genetic distance with the SMV isolate from Jiangsu province (accession number: MH919386), clustering with SMV isolates from Jiangsu, Zhejiang and Shanxi provinces. Similarly, the nucleotide sequences of SMV-Gm2, SMV-Gm4, and SMV-Gm6 exhibited the highest similarity (98.48% to 98.51%) with the SMV isolate from South Korea (accession number: FJ640954), indicating a close genetic relationship. |
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