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乔 蕊, 周雪平*, 李方方*.在中国广东侵染胜红蓟的中国胜红蓟黄脉病毒的鉴定和全基因组分析[J].植物保护,2024,50(3):266-271.
在中国广东侵染胜红蓟的中国胜红蓟黄脉病毒的鉴定和全基因组分析
Identification and complete genome sequence analysis of ageratum-infecting ageratum yellow vein China virus from Guangdong, China
投稿时间:2023-05-05  修订日期:2023-07-05
DOI:10.16688/j.zwbh.2023216
中文关键词:  中国胜红蓟黄脉病毒  PCR技术  基因组序列  系统发育分析
英文关键词:ageratum yellow vein China virus  PCR technology  genome sequence  phylogeny analysis
基金项目:国家自然科学基金(32172385)
作者单位E-mail
乔 蕊, 周雪平*, 李方方* 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193 周雪平zzhou@zju.edu.cn; 李方方lifangfang@caas.cn 
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中文摘要:
      2022年9月在广东省罗定市发现了叶片表现为黄色网状症状的胜红蓟病株。为了明确胜红蓟叶片的黄脉症状是否由双生病毒感染引起, 本研究使用检测双生病毒的简并引物PA/PB进行PCR扩增, 获得约500 bp的片段。根据该序列设计特异性引物扩增并且克隆得到了病毒DNA-A的全基因组序列。通过BLAST比对发现, 获得的DNA-A与中国胜红蓟黄脉病毒(ageratum yellow vein China virus, AYVCNV)海南分离物(OQ421190)的DNA-A的相似性最高, 相似度为98.11%。系统进化树分析显示, 获得的病毒DNA-A与海南分离物(OQ421190)在同一分支, 说明具有较近的亲缘关系。以上研究结果表明侵染胜红蓟的病毒是AYVCNV的分离物。这是关于AYVCNV在广东地区侵染胜红蓟的首次报道, 可为当地病毒病的防控提供参考。
英文摘要:
      The ageratum plants with yellow vein symptoms were found in Luoding city, Guangdong province, in September 2022. To determine whether the ageratum plants showing vein yellowing symptoms were caused by geminiviruses, PCR technology was used to amplify about 500 bp sequence using the degenerate primers PA/PB for detecting the presence of geminiviruses in this study. Based on the amplified 500 bp sequence, the complete genome sequences of DNA-A component were obtained by PCR amplification, cloning, and sequencing. BLAST comparison showed that the DNA-A component shared the highest sequence identity (98.11%) with the Hainan isolate of ageratum yellow vein China virus (OQ421190). Phylogeny analysis showed that the complete genome sequence of DNA-A component was most closely related to the Hainan isolate of AYVCNV (OQ421190). These results suggest that the virus infecting ageratum is an isolate of AYVCNV. This is the first report on AYVCNV infecting ageratum in Guangdong, which can provide a reference for local virus disease prevention and control.
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