• 首页关于本刊投稿须知订阅指南广告合作投稿指南旧版入口联系我们
期刊订阅

植物保护淘宝

植物保护微店
邱琦珍1, 2, 潘兴鲁2, 吴小虎2, 徐 军2, 董丰收1, 2*, 郑永权2.超高效液相色谱串联质谱法测定豇豆中新烟碱类农药残留[J].植物保护,2024,50(3):247-252.
超高效液相色谱串联质谱法测定豇豆中新烟碱类农药残留
Determination of residues of neonicotinoid insecticides in cowpeas by UPLC-MS/MS
投稿时间:2023-04-14  修订日期:2023-05-02
DOI:10.16688/j.zwbh.2023175
中文关键词:  新烟碱类杀虫剂  豇豆  超高效液相色谱-串联质谱  残留检测
英文关键词:neonicotinoid insecticide  cowpea  UPLC-MS/MS  residue detection
基金项目:国家自然科学基金(31872004)
作者单位E-mail
邱琦珍1, 2, 潘兴鲁2, 吴小虎2, 徐 军2, 董丰收1, 2*, 郑永权2 1. 天津农学院园艺园林学院, 天津 300384
2. 中国农业科学院植物保护研究所, 植物病虫害综合治理全国重点实验室, 北京 100193 
dongfengshou@caas.cn 
摘要点击次数: 38
全文下载次数: 52
中文摘要:
      本研究建立了超高效液相色谱-串联质谱法(UPLC-MS/MS)同时检测豇豆中9种新烟碱类杀虫剂的多残留分析方法。豇豆样品用QuEChERS方法前处理, 用Kinetex Biphenyl色谱柱分离, 以甲醇和含0.1 mmol/L甲酸铵、0.001%甲酸的水作为流动相进行梯度洗脱, 使用超高效液相色谱串联质谱检测分析。定量限为0.01 mg/kg, 标准工作曲线在0.001~1 mg/kg范围内表现出良好的线性关系, 决定系数(R2)均大于0.99。目标杀虫剂在豇豆中的平均回收率为66.9%~109.8%, 相对标准偏差为1.2%~10.0%。应用该方法对某地16个农贸市场中采集的71份豇豆样本进行检测, 所有样品均符合我国所规定的豇豆(或豆类)中的最大残留限量标准要求。该方法简便、准确、灵敏度高, 适用于新烟碱类杀虫剂在豇豆中的残留监测。
英文摘要:
      A method was developed for the simultaneous determination of nine neonicotinoid insecticides in cowpea by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The cowpea samples were pretreated with QuEChERS, and separation was performed by Kinetex Biphenyl column using a gradient elution of methanol and water (0.1 mmol/L ammonium formate+0.001% formic acid) as mobile phases, and then detected by UPLC-MS/MS. The limit of quantification (LOQ) for neonicotinoid insecticides was 0.01 mg/kg. The method showed excellent linearity (R2>0.99) in the range of 0.001-1 mg/kg. The average recoveries of the target compounds in cowpea ranged from 66.9% to 109.8%, and the relative standard deviation (RSDs) was from 1.2% to 10.0%. This method was used for determination of 71 cowpea samples collected in 16 markets and all pesticides met the requirements of the maximum residue limit standard in cowpeas (or beans) stipulated in the National Food Safety Standard Maximum Residue Limits of Pesticides in Food (GB 2763-2021). This method is simple, accurate and sensitive, and suitable for the residue analysis of neonicotinoid insecticides in cowpeas.
查看全文  查看/发表评论  下载PDF阅读器
关闭