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陈实锐1, 陈哲晶1, 郑欣然1, 李佳灵2, 张贝贝1*.琼凹大叶蝉精子发生及精子超微结构[J].植物保护,2024,50(3):99-110.
琼凹大叶蝉精子发生及精子超微结构
Spermatogenesis and sperm ultrastructure of Bothrogonia qiongana Yang et Li (Hemiptera: Cicadellidae)
投稿时间:2023-06-02  修订日期:2023-10-12
DOI:10.16688/j.zwbh.2023271
中文关键词:  头喙亚目  大叶蝉亚科  生殖细胞  精子形成  超微结构  副体
英文关键词:Auchenorrhyncha  Cicadellinae  germ cell  spermiogenesis  ultrastructure  accessory body
基金项目:国家自然科学基金(31960109);海南省自然科学基金(319QN170)
作者单位E-mail
陈实锐1, 陈哲晶1, 郑欣然1, 李佳灵2, 张贝贝1* 1. 海南大学林学院, 儋州 571737
2. 海南热带雨林国家公园管理局五指山分局, 五指山 572200 
zhangbeibei@hainanu.edu.cn 
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中文摘要:
      昆虫精子超微结构在不同类群间变异较大, 在昆虫分类、种群进化研究及系统发育分析中具有重要意义。研究叶蝉科昆虫精子发生及精子超微结构, 可为叶蝉科及头喙亚目系统发育分析提供更多特征依据。本研究采用光学和电子显微镜技术, 观察了琼凹大叶蝉Bothrogonia qiongana Yang et Li的精子发生和精子超微结构。精子形成时期, 精子细胞内高尔基复合体形成精子顶体; 线粒体融合衍变为线粒体衍生物; 中心粒产生轴丝。单层微管结构围绕精子各组成部分, 在精子成熟后消失, 对精子细胞伸长起关键作用。成熟精子细长、线状, 头部顶端由绳状物质连接形成梳状精子束。顶体圆锥状, 具有顶体腔, 末端延伸出2条突起插入细胞核顶端。细胞核近柱状, 末端平截, 侧缘凹陷, 通过片层状中心粒侧体与鞭毛线粒体衍生物连接。尾部鞭毛由1条9+9+2型轴丝, 2条对称线粒体衍生物, 2条较短副体, 2条细小附结构及鞭毛中心物质组成。琼凹大叶蝉副体横切椭圆形、长度较短仅出现在鞭毛起始区, 与角顶叶蝉亚科昆虫差异较大。结合已报道头喙亚目类昆虫, 我们推断副体结构可为叶蝉科及头喙亚目系统发育分析提供有用的特征。
英文摘要:
      Sperm ultrastructure displays a great variability among insect groups and holds great significance in the classification, evolution, and phylogenetic analysis of insects. The study of spermatogenesis and sperm ultrastructure in Cicadellidae can provide additional valuable characters for phylogenetic analysis of both Cicadellidae and Auchenorrhyncha. Spermatogenesis and sperm ultrastructure of the leafhopper Bothrogonia qiongana Yang et Li were investigated using light and electron microscopy. During spermiogenesis, the acrosome originated from the Golgi apparatus, while the mitochondrial derivatives formed through the aggregation and fusion of numerous mitochondria in the spermatid cytoplasm. The flagellar axoneme was generated by the centriole. Spermatid components were surrounded by a single layer of microtubules, which played a critical role in the spermatid elongation during spermiogenesis, which then disappeared in mature spermatozoa. The spermatozoa exhibited an elongated, filiform structure. The sperm heads were arranged in a row with a rope-like structure, forming a pectinate sperm bundle. The conical acrosome possessed a sub-acrosomal space and formed two acrosomal processes inserting into both sides of the anterior part of nucleus. The nucleus was cylindrical, with a flattened base, and its marginal depression connected to the two mitochondrial derivatives of the flagellum through a lamellate centriolar adjunct. The flagellum was composed of a 9+9+2 axoneme, two symmetrical mitochondrial derivatives, two short accessory bodies, two small sub-ellipsoidal structures, and a center-flagellar material. Two ovoid and short accessory bodies observed only in the flagellum’s initial region of Bothrogonia qiongana, were quite different from those of species in Deltocephalinae. Combined with the reported data from other auchenorrhynchans, these accessory bodies likely provide valuable characters for phylogenetic analysis of Cicadellidae and Auchenorrhyncha.
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